AAV VIRION WITH DECREASED IMMUNOREACTIVITY AND USE THEREOF

To provide an AAV variant with decreased immunoreactivity.SOLUTION: There are disclosed a method for preparing recombinant AAV virion with decreased immunoreactivity and a method for using the same. The recombinant AAV virion includes mutated capsid proteins or is derived from non-primate mammalian...

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Bibliographische Detailangaben
Hauptverfasser:	ARBETMAN ALEJANDRA ELENA, LOCHRIE MICHAEL A, SUROSKY RICHARD T, COLOSI PETER
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide an AAV variant with decreased immunoreactivity.SOLUTION: There are disclosed a method for preparing recombinant AAV virion with decreased immunoreactivity and a method for using the same. The recombinant AAV virion includes mutated capsid proteins or is derived from non-primate mammalian AAV serotypes and isolates that display decreased immunoreactivity against AAV-2. There are provided, in one embodiment, a method for effective delivery of a heterologous nucleic acid molecule (HNA) to cells or tissues of a vertebrate subject (for example, a mammalian) using the recombinant AAV virion, and an AAV vector used in the method.SELECTED DRAWING: Figure 1 【課題】免疫反応性が減少したＡＡＶ変異体を提供する。【解決手段】免疫反応性が減少した組み換え型ＡＡＶビリオンの作製方法および使用方法が記載される。組み換え型ＡＡＶビリオンは、変異型キャプシドタンパク質を含むか、または、ＡＡＶ－２に対して減少した免疫反応性を示す非霊長類の哺乳動物ＡＡＶ血清型および単離体に由来する。１実施形態において、脊椎動物被験体（例えば、哺乳動物）の細胞または組織への、組み換え型ＡＡＶビリオンを用いた、異種性の核酸分子（ＨＮＡ）の効率的な送達のための方法、および、この方法において使用するためのＡＡＶベクターが提供される。【選択図】図１