PCR REACTION VESSEL AND PCR DEVICE

To prevent contamination of a PCR reaction vessel.SOLUTION: A PCR reaction vessel 10 comprises: a substrate 14; a flow channel 12 formed on the substrate 14; a pair of first filter 28 and second filter 30 provided on both ends of the flow channel 12; a pair of first air communication port 24 and sec...

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Bibliographische Detailangaben
Hauptverfasser: NAGAI SHUSUKE, NISHIZAWA NAOFUMI, FUKUZAWA TAKASHI
Format: Patent
Sprache:eng ; jpn
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Zusammenfassung:To prevent contamination of a PCR reaction vessel.SOLUTION: A PCR reaction vessel 10 comprises: a substrate 14; a flow channel 12 formed on the substrate 14; a pair of first filter 28 and second filter 30 provided on both ends of the flow channel 12; a pair of first air communication port 24 and second air communication port 26 which communicate with the flow channel 12 through the first filter 28 and the second filter 30; a thermal cycle region 12e formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch point 112c formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch flow channel 131 whose one end is connected to the branch point 112c; and a sample introduction port 133 formed on the other end of the branch flow channel 131.SELECTED DRAWING: Figure 1 【課題】PCR反応容器のコンタミネーションを防止する。【解決手段】PCR反応容器10は、基板14と、基板14に形成された流路12と、流路12の両端に設けられた一対の第1フィルタ28および第2フィルタ30と、第1フィルタ28および第2フィルタ30を通じて流路12と連通する一対の第1空気連通口24および第2空気連通口26と、流路12における第1フィルタ28および第2フィルタ30の間に形成されたサーマルサイクル領域12eと、流路12における第1フィルタ28および第2フィルタ30の間に形成された分岐点112cと、一端が分岐点112cに接続された分岐流路131と、分岐流路131の他端に形成された試料導入口133とを備える。【選択図】図1