METHODS OF SEQUENCING NUCLEIC ACIDS IN MIXTURES AND COMPOSITIONS RELATED THERETO

To provide methods of sequencing nucleic acids in mixtures and compositions related thereto.SOLUTION: This disclosure relates to a process of analyzing the end-to-end sequence and a process of analyzing the relative distributions in heterogeneous mixtures of polynucleotides, and methods and enabling...

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Bibliographische Detailangaben
Hauptverfasser:	WILLIAM S AGNEW, MARK C EMERICK
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide methods of sequencing nucleic acids in mixtures and compositions related thereto.SOLUTION: This disclosure relates to a process of analyzing the end-to-end sequence and a process of analyzing the relative distributions in heterogeneous mixtures of polynucleotides, and methods and enabling reagents related thereto. In certain embodiments, this method relates to the complete full-length sequencing and quantitative profiling of mRNAs present in the transcriptomes of cells or tissues of, but not limited to, higher multicellular organisms that possess interrupted genes subjected to complex post-transcriptional RNA processing.SELECTED DRAWING: None 【課題】混合物中の核酸を配列決定する方法およびそれに関する組成物の提供。【解決手段】本開示は、端から端までの配列を分析する工程およびポリヌクレオチドの異種混合物中の相対的分配を分析する工程、ならびにこれに関する方法およびこれを可能にする試薬に関する。ある種の実施形態において、この方法は、複雑な転写後ＲＮＡプロセシングに供される分断遺伝子を有する高等な多細胞生物（これに限定されない）の細胞もしくは組織のトランスクリプトームに存在するｍＲＮＡの完全な全長配列決定および定量的プロファイリングに関する。【選択図】なし