USE OF BIOLOGICAL RNA SCAFFOLDS WITH IN VITRO SELECTION TO GENERATE ROBUST SMALL MOLECULE BINDING APTAMERS FOR GENETICALLY ENCODABLE BIOSENSORS

To provide libraries of scaffolds derived from riboswitches and small ribozymes and their methods of use.SOLUTION: Scaffolds of the present invention yield aptamers that are easily identified and characterized by virtue of the structural scaffold. The nature of the scaffold predisposes these RNAs fo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ELY B PORTER, ROBERT T BATEY
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	To provide libraries of scaffolds derived from riboswitches and small ribozymes and their methods of use.SOLUTION: Scaffolds of the present invention yield aptamers that are easily identified and characterized by virtue of the structural scaffold. The nature of the scaffold predisposes these RNAs for coupling to readout domains to engineer biosensors that function in vitro and in vivo. Biosensors, synthetic RNA agents and synthetic DNA agents, and their methods of use, are also provided.SELECTED DRAWING: None 【課題】リボスイッチ及び小リボザイム由来の足場のライブラリならびにそれらの使用方法が本明細書に提供される。【解決手段】本発明の足場は、構造的足場により容易に特定及び特徴付けされるアプタマーをもたらす。足場の性質は、読み取りドメインに結合するためのこれらのＲＮＡが、インビトロ及びインビボで機能するバイオセンサーを操作しやすくなることである。バイオセンサー、合成ＲＮＡ剤、及び合成ＤＮＡ剤、ならびにそれらの使用方法も提供される。【選択図】なし