METHOD FOR MEASURING INTERCELLULAR BOND, FUSION, OR THEIR INHIBITORY ACTIVITY THROUGH PROTEINS EXPRESSED ON CELL SURFACES

To provide a method that can quantitatively measure bonding between proteins expressed on cell membranes in a shorter time.SOLUTION: The present invention provides a method for measuring intercellular bonding through proteins expressed on cell surfaces, including the steps of: culturing cells expres...

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Bibliographische Detailangaben
Hauptverfasser: WATANABE SHINYA, HOSHI HIROTAKA, SATO SHIGEKO, IMAI JUNICHI
Format: Patent
Sprache:eng ; jpn
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Zusammenfassung:To provide a method that can quantitatively measure bonding between proteins expressed on cell membranes in a shorter time.SOLUTION: The present invention provides a method for measuring intercellular bonding through proteins expressed on cell surfaces, including the steps of: culturing cells expressing membrane proteins and cells expressing proteins bound to the membrane proteins on cell surfaces; and measuring the intercellular bonding state after the culturing step.SELECTED DRAWING: None 【課題】 本発明は細胞膜上に発現するタンパク質同士の結合をより短時間で定量的に測定できる手法の提供を課題とする。【解決手段】 細胞表面に発現するタンパク質を介した細胞同士の結合を測定する方法であって、膜タンパク質を発現する細胞と、膜タンパク質に結合するタンパク質を細胞表面に発現する細胞とを培養する工程と、培養工程後の細胞同士の結合状態を測定する工程と含む、測定方法。【選択図】なし