GENETICALLY MODIFIED MICROORGANISM HAVING ISOPRENOIDS PRODUCING ABILITY, AND PRODUCTION METHOD OF ISOPRENOIDS USING THE SAME

To provide a technique for producing bio-isoprene by using a microorganism as a host.SOLUTION: A genetically modified microorganism having isoprenoids producing ability has phospho glucose isomerase (Pgi) activity which is reduced compared to an unmodified body, and 1-deoxy-D-xylulose 5- phosphoric...

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Hauptverfasser:	SAKAMOTO TAKESHI, NAKAJIMA NOBUTAKA, YUMURA SHUICHI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a technique for producing bio-isoprene by using a microorganism as a host.SOLUTION: A genetically modified microorganism having isoprenoids producing ability has phospho glucose isomerase (Pgi) activity which is reduced compared to an unmodified body, and 1-deoxy-D-xylulose 5- phosphoric acid synthase (Dxs) activity, isopentyl diphosphoric acid isomerase (Idi) activity, and isoprene synthase (IspS) activity which are enhanced compared to an unmodified body, and further has 1-deoxy-D-xylulose 5-phosphoric acid reductoisomerase (Dxr) activity, 1-hydroxy-2-methyl-2-butenyl 4-diphosphoric acid synthase (IspG) activity and/or 1-hydroxy-2-methyl-2-butenyl 4-diphosphoric acid reductase (IspH) activity, ferredoxin/flavodoxin (petF/fldA) activity, and ferredoxin/flavodoxin-NADP reductase (petH/fpr) activity which are enhanced compared to an unmodified body.SELECTED DRAWING: Figure 2 【課題】微生物を宿主としてバイオイソプレンを生産するための技術の提供。【解決手段】非改変体に比較して低減されたホスホグルコースイソメラーゼ（Pgi）活性と、非改変体に比較して増強された１－デオキシ－Ｄ－キシルロース５－リン酸シンターゼ（Dxs）活性、イソペンチル二リン酸イソメラーゼ（Idi）活性、およびイソプレンシンターゼ（IspS）活性を有し、さらに、非改変体に比較して増強された１－デオキシ－Ｄ－キシルロース５－リン酸レダクトイソメラーゼ（Dxr）活性、１－ヒドロキシ－２－メチル－２－ブテニル４－二リン酸シンターゼ（IspG）活性および／または１－ヒドロキシ－２－メチル－２－ブテニル４－二リン酸レダクターゼ（IspH）活性、フェレドキシン／フラボドキシン（petF/fldA）活性、およびフェレドキシン／フラボドキシン-NADPレダクターゼ（petH/fpr）活性を有する、イソプレノイド類産生能を有する遺伝子改変微生物を提供する。【選択図】図２