COMPOSITION FOR IMPROVING MOLECULAR BARCODING EFFICIENCY AND USES THEREOF

To provide compositions for improving molecular barcoding efficiency and to provide uses thereof.SOLUTION: The present invention relates to a composition for improving molecular barcoding efficiency and to uses thereof, and provides a method for producing a DNA library, molecular barcoding methods f...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	KIM SOL YI, PARK DONG HYUN, LIM HYEYEUN, PARK HYEONG BEEN, CHUNG JONG SUK, SHIN SEUNG HO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide compositions for improving molecular barcoding efficiency and to provide uses thereof.SOLUTION: The present invention relates to a composition for improving molecular barcoding efficiency and to uses thereof, and provides a method for producing a DNA library, molecular barcoding methods for nucleic acid sequence analysis, and a composition for DNA library production, according to the method and the composition, by using a barcode sequence that is arranged to include the first nucleotide position in an adapter based on the terminal region of the adapter that is ligated with a DNA fragment, a molecular barcoding alternation phenomenon being reduced and the accuracy of a nucleic acid sequence analysis being improved.SELECTED DRAWING: Figure 11 【課題】分子バーコディング効率を向上させるための組成物及びその用途を提供する。【解決手段】分子バーコディング効率を向上させるための組成物及びその用途に係り、ＤＮＡライブラリを製造する方法、核酸配列分析のための分子バーコディング方法、及びＤＮＡライブラリ製造用組成物を提供し、該方法及び該組成物によれば、ＤＮＡ切片とライゲーションされるアダプタの末端領域を基準に、アダプタ内最初ヌクレオチド位置を含むように配列されるバーコード配列を利用することにより、分子バーコディング交替現象を低減させ、核酸配列分析の正確度を向上させる。【選択図】図１１