MEASUREMENT METHOD OF THE NUMBER OF LIVING CELLS, AND MEASUREMENT DEVICE OF THE NUMBER OF LIVING CELLS

To provide a measurement method of the number of living cells and a measurement device of the number of living cells which cause little risk of contamination, and can measure the number of living cells highly accurately by non-invasive means.SOLUTION: A measurement method of the number of living cells includes the steps of: measuring the amount of suspensoid included in cell suspension; measuring the capacitance of the cell suspension which varies due to a life-and-death state of a cell; calculating one or more of the number of living cells, the number of dead cells and the survival rate of cells included in the cell suspension on the basis of the previously obtained capacitance per unit living cell, the previously obtained capacitance per unit dead cell, the amount of the suspensoid, and the capacitance of the cell suspension. The number of living cells measurement device 1 is equipped with: a turbidity sensor 2; a measuring device 3 which measures the capacitance of cell suspension; a storage unit which stores data of the capacitance per unit living cell and the capacitance per unit dead cell; and a calculation unit.SELECTED DRAWING: Figure 1 【課題】コンタミネーションのリスクが少なく、非侵襲的な手段で生細胞数を高精度に計測することができる生細胞数計測方法および生細胞数計測装置を提供する。【解決手段】生細胞数計測方法は、細胞懸濁液に含まれる懸濁質の量を測定する工程と、細胞の生死の状態によって変化する前記細胞懸濁液のキャパシタンスを測定する工程と、予め求められた単位生細胞当たりのキャパシタンス、予め求められた単位死細胞当たりのキャパシタンス、前記懸濁質の量、および、前記細胞懸濁液のキャパシタンスに基づいて、前記細胞懸濁液に含まれる生細胞数、死細胞数、および、細胞の生存率のうちの一以上を演算する工程と、を含む。生細胞数計測装置１は、濁度センサ２と、細胞懸濁液のキャパシタンスを測定する測定器３と、単位生細胞当たりのキャパシタンスと単位死細胞当たりのキャパシタンスのデータを記憶した記憶部と、演算部とを備える。【選択図】図１