METHOD FOR MANUFACTURING DEVICE, DEVICE, AND KIT
To provide a method for manufacturing a device, which allows a known quantity of a reagent to be reliably immobilized in a reaction field, which can be stored at room temperature, and which enables the performance of a real-time PCR apparatus to be correctly evaluated.SOLUTION: The present invention...
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| creator | UNNO HIROTAKA YONEKAWA YUKI NUNOFUJI SATOSHI HASHIMOTO MICHIE ONISHI MARI NAKAZAWA SATOSHI RIZTYAN |
| description | To provide a method for manufacturing a device, which allows a known quantity of a reagent to be reliably immobilized in a reaction field, which can be stored at room temperature, and which enables the performance of a real-time PCR apparatus to be correctly evaluated.SOLUTION: The present invention discloses a method for manufacturing a device having at least one well, in which a specific number of copies of a nucleic acid in the at least one well are immobilized in a reaction field. The method includes a nucleic acid extraction step for extracting the nucleic acid with an enzyme and a drying deactivation step for deactivating the enzyme by drying at 5°C to 45°C.SELECTED DRAWING: None
【課題】既知数の試薬が確実に反応場に固定され、常温で保存可能であり、且つ、リアルタイムＰＣＲ装置の性能を正しく評価できるデバイスの製造方法を提供する。【解決手段】少なくとも１つのウェルを有し、少なくとも１つの前記ウェル内の核酸が、反応場に特定コピー数固定されているデバイスの製造方法であって、前記核酸を酵素により抽出する核酸抽出工程、及び、前記酵素を５～４５℃で乾燥させることにより失活させる乾燥失活工程を含む、デバイスの製造方法。【選択図】なし |
| format | Patent |
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【課題】既知数の試薬が確実に反応場に固定され、常温で保存可能であり、且つ、リアルタイムＰＣＲ装置の性能を正しく評価できるデバイスの製造方法を提供する。【解決手段】少なくとも１つのウェルを有し、少なくとも１つの前記ウェル内の核酸が、反応場に特定コピー数固定されているデバイスの製造方法であって、前記核酸を酵素により抽出する核酸抽出工程、及び、前記酵素を５～４５℃で乾燥させることにより失活させる乾燥失活工程を含む、デバイスの製造方法。【選択図】なし</description><language>eng ; jpn</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220609&DB=EPODOC&CC=JP&NR=2022086982A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25547,76298</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220609&DB=EPODOC&CC=JP&NR=2022086982A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>UNNO HIROTAKA</creatorcontrib><creatorcontrib>YONEKAWA YUKI</creatorcontrib><creatorcontrib>NUNOFUJI SATOSHI</creatorcontrib><creatorcontrib>HASHIMOTO MICHIE</creatorcontrib><creatorcontrib>ONISHI MARI</creatorcontrib><creatorcontrib>NAKAZAWA SATOSHI</creatorcontrib><creatorcontrib>RIZTYAN</creatorcontrib><title>METHOD FOR MANUFACTURING DEVICE, DEVICE, AND KIT</title><description>To provide a method for manufacturing a device, which allows a known quantity of a reagent to be reliably immobilized in a reaction field, which can be stored at room temperature, and which enables the performance of a real-time PCR apparatus to be correctly evaluated.SOLUTION: The present invention discloses a method for manufacturing a device having at least one well, in which a specific number of copies of a nucleic acid in the at least one well are immobilized in a reaction field. The method includes a nucleic acid extraction step for extracting the nucleic acid with an enzyme and a drying deactivation step for deactivating the enzyme by drying at 5°C to 45°C.SELECTED DRAWING: None
【課題】既知数の試薬が確実に反応場に固定され、常温で保存可能であり、且つ、リアルタイムＰＣＲ装置の性能を正しく評価できるデバイスの製造方法を提供する。【解決手段】少なくとも１つのウェルを有し、少なくとも１つの前記ウェル内の核酸が、反応場に特定コピー数固定されているデバイスの製造方法であって、前記核酸を酵素により抽出する核酸抽出工程、及び、前記酵素を５～４５℃で乾燥させることにより失活させる乾燥失活工程を含む、デバイスの製造方法。【選択図】なし</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDDwdQ3x8HdRcPMPUvB19At1c3QOCQ3y9HNXcHEN83R21YHTjn4uCt6eITwMrGmJOcWpvFCam0HJzTXE2UM3tSA_PrW4IDE5NS-1JN4rwMjAyMjAwszSwsjRmChFAEiPJkg</recordid><startdate>20220609</startdate><enddate>20220609</enddate><creator>UNNO HIROTAKA</creator><creator>YONEKAWA YUKI</creator><creator>NUNOFUJI SATOSHI</creator><creator>HASHIMOTO MICHIE</creator><creator>ONISHI MARI</creator><creator>NAKAZAWA SATOSHI</creator><creator>RIZTYAN</creator><scope>EVB</scope></search><sort><creationdate>20220609</creationdate><title>METHOD FOR MANUFACTURING DEVICE, DEVICE, AND KIT</title><author>UNNO HIROTAKA ; YONEKAWA YUKI ; NUNOFUJI SATOSHI ; HASHIMOTO MICHIE ; ONISHI MARI ; NAKAZAWA SATOSHI ; RIZTYAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2022086982A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2022</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>UNNO HIROTAKA</creatorcontrib><creatorcontrib>YONEKAWA YUKI</creatorcontrib><creatorcontrib>NUNOFUJI SATOSHI</creatorcontrib><creatorcontrib>HASHIMOTO MICHIE</creatorcontrib><creatorcontrib>ONISHI MARI</creatorcontrib><creatorcontrib>NAKAZAWA SATOSHI</creatorcontrib><creatorcontrib>RIZTYAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>UNNO HIROTAKA</au><au>YONEKAWA YUKI</au><au>NUNOFUJI SATOSHI</au><au>HASHIMOTO MICHIE</au><au>ONISHI MARI</au><au>NAKAZAWA SATOSHI</au><au>RIZTYAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR MANUFACTURING DEVICE, DEVICE, AND KIT</title><date>2022-06-09</date><risdate>2022</risdate><abstract>To provide a method for manufacturing a device, which allows a known quantity of a reagent to be reliably immobilized in a reaction field, which can be stored at room temperature, and which enables the performance of a real-time PCR apparatus to be correctly evaluated.SOLUTION: The present invention discloses a method for manufacturing a device having at least one well, in which a specific number of copies of a nucleic acid in the at least one well are immobilized in a reaction field. The method includes a nucleic acid extraction step for extracting the nucleic acid with an enzyme and a drying deactivation step for deactivating the enzyme by drying at 5°C to 45°C.SELECTED DRAWING: None
【課題】既知数の試薬が確実に反応場に固定され、常温で保存可能であり、且つ、リアルタイムＰＣＲ装置の性能を正しく評価できるデバイスの製造方法を提供する。【解決手段】少なくとも１つのウェルを有し、少なくとも１つの前記ウェル内の核酸が、反応場に特定コピー数固定されているデバイスの製造方法であって、前記核酸を酵素により抽出する核酸抽出工程、及び、前記酵素を５～４５℃で乾燥させることにより失活させる乾燥失活工程を含む、デバイスの製造方法。【選択図】なし</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD FOR MANUFACTURING DEVICE, DEVICE, AND KIT |
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