METHOD FOR MANUFACTURING DEVICE, DEVICE, AND KIT

To provide a method for manufacturing a device, which allows a known quantity of a reagent to be reliably immobilized in a reaction field, which can be stored at room temperature, and which enables the performance of a real-time PCR apparatus to be correctly evaluated.SOLUTION: The present invention...

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Bibliographische Detailangaben
Hauptverfasser:	UNNO HIROTAKA, YONEKAWA YUKI, NUNOFUJI SATOSHI, HASHIMOTO MICHIE, ONISHI MARI, NAKAZAWA SATOSHI, RIZTYAN
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a method for manufacturing a device, which allows a known quantity of a reagent to be reliably immobilized in a reaction field, which can be stored at room temperature, and which enables the performance of a real-time PCR apparatus to be correctly evaluated.SOLUTION: The present invention discloses a method for manufacturing a device having at least one well, in which a specific number of copies of a nucleic acid in the at least one well are immobilized in a reaction field. The method includes a nucleic acid extraction step for extracting the nucleic acid with an enzyme and a drying deactivation step for deactivating the enzyme by drying at 5°C to 45°C.SELECTED DRAWING: None 【課題】既知数の試薬が確実に反応場に固定され、常温で保存可能であり、且つ、リアルタイムＰＣＲ装置の性能を正しく評価できるデバイスの製造方法を提供する。【解決手段】少なくとも１つのウェルを有し、少なくとも１つの前記ウェル内の核酸が、反応場に特定コピー数固定されているデバイスの製造方法であって、前記核酸を酵素により抽出する核酸抽出工程、及び、前記酵素を５～４５℃で乾燥させることにより失活させる乾燥失活工程を含む、デバイスの製造方法。【選択図】なし