PURIFICATION METHODS FOR RECOMBINANTLY-PRODUCED RSV IN TRIMERIC FORM

To provide methods for purifying recombinantly produced trimer typed RSV proteins.SOLUTION: The invention relates to a purification method of a recombinantly-produced RSV F protein in trimeric form. According to the invention, the method sequentially comprises an anion exchange chromatography step,...

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Bibliographische Detailangaben
Hauptverfasser:	EUGENE JOSEPH VIDUNAS, ZHAO JAY ZHIXING, MICHELE L WEAVER, KOH EUN HEE, YUAN YONGHUI, YE XINHAO, CAI PING
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide methods for purifying recombinantly produced trimer typed RSV proteins.SOLUTION: The invention relates to a purification method of a recombinantly-produced RSV F protein in trimeric form. According to the invention, the method sequentially comprises an anion exchange chromatography step, a cHA chromatography step and a HIC step. The invention is also directed to a pharmaceutical product including an RSV F protein purified by such a method.SELECTED DRAWING: Figure 1 【課題】本発明は、組換え生産された三量体型のＲＳＶＦタンパク質の精製方法に関する。【解決手段】本発明によれば、方法は、陰イオン交換クロマトグラフィーステップ、ｃＨＡクロマトグラフィーステップ、およびＨＩＣステップを順次含む。本発明はまた、このような方法によって精製されたＲＳＶＦタンパク質を含む医薬製品も対象とする。【選択図】図１