MODIFIED PIGGYBAC TRANSPOSASE POLYPEPTIDE, POLYNUCLEOTIDE ENCODING THE SAME, INTRODUCTORY CARRIER, KIT, METHOD FOR INTEGRATING TARGET SEQUENCE INTO CELLULAR GENOME, AND CELL MANUFACTURING METHOD

To provide a modified piggyBac transposase polypeptide that can more efficiently integrate the target sequence into the cellular genome, a polynucleotide encoding it, an introductory carrier, kit, a method for integrating the target sequence into the cellular genome, and a cell manufacturing method....

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Hauptverfasser:	AKABOSHI HIDEKAZU, NOZAKI EMI, ISHIHARA MITSUKO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a modified piggyBac transposase polypeptide that can more efficiently integrate the target sequence into the cellular genome, a polynucleotide encoding it, an introductory carrier, kit, a method for integrating the target sequence into the cellular genome, and a cell manufacturing method.SOLUTION: A modified piggyBac transposase polypeptide according to an embodiment comprises an amino acid sequence of piggyBac transposase and an amino acid sequence of a nuclear localization signal.SELECTED DRAWING: Figure 1 【課題】本発明が解決しようとする課題は、より効率的に細胞のゲノムに目的配列を組込むことができる改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法を提供することである。【解決手段】実施形態に従う改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチドは、ｐｉｇｇｙＢａｃトランスポゼースのアミノ酸配列と、核局在化シグナルのアミノ酸配列とを含む。【選択図】図１