METHOD FOR PRODUCING TARGET CELL, METHOD FOR PRODUCING PRODUCT WITH TARGET CELL, AND SERUM-FREE MEDIUM

To provide a cell culture technique that uses a serum substitute, can maintain cell culture efficiency when using the serum, and can solve the problem of cost when using the serum and the problem of quality stability of cultured cells.SOLUTION: The present invention discloses a method for producing...

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Hauptverfasser:	MOGI ITSURO, MINAMI KAZUNARI, ETO HIROYUKI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a cell culture technique that uses a serum substitute, can maintain cell culture efficiency when using the serum, and can solve the problem of cost when using the serum and the problem of quality stability of cultured cells.SOLUTION: The present invention discloses a method for producing a target cell by culturing a source cell in a serum-free medium containing at least one culture factor selected from a growth factor and a differentiation induction factor, human serum albumin, and at least one additive selected from the group consisting of Src inhibitor, PKC activator, methyl cellulose, Essential 8 medium, recombinant human serum albumin, and non-human animal serum albumin.SELECTED DRAWING: None 【課題】血清代替物を使用した細胞培養技術であって、血清使用時の細胞の培養効率を維持することができるとともに、血清使用時のコストの問題や培養細胞の品質安定性の問題を解決することができる細胞培養技術を提供すること。【解決手段】増殖因子および分化誘導因子から選択される少なくとも１つの培養因子と、ヒト血清アルブミンと、Ｓｒｃ阻害剤、ＰＫＣ活性化剤、メチルセルロース、Essential 8培地、組換えヒト血清アルブミン、およびヒト以外の動物血清アルブミンからなる群より選択される少なくとも１つの添加物とを含む無血清培地で原料細胞を培養して目的細胞を生産することを含む、目的細胞の生産方法。【選択図】なし