DNA POLYMERASE MUTANTS HAVING ENHANCED TEMPLATE DISCRIMINATION ACTIVITY

To provide mutant DNA polymerases having enhanced primer and/or template discrimination activity, and methods for polymerase-based assays for genetic diagnostic analysis.SOLUTION: A mutant Taq DNA polymerase having an enhanced template discrimination activity compared with an unmodified Taq DNA poly...

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Hauptverfasser:	SUSAN M RUPP, MARK AARON BEHLKE, RICHARD OWCZARZY, SCOTT D ROSE, JOSEPH R DOBOSY, JOSEPH A WALDER
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide mutant DNA polymerases having enhanced primer and/or template discrimination activity, and methods for polymerase-based assays for genetic diagnostic analysis.SOLUTION: A mutant Taq DNA polymerase having an enhanced template discrimination activity compared with an unmodified Taq DNA polymerase, where the amino acid sequence of the mutant Taq DNA polymerase includes a substitution at specific residue positions of the unmodified Taq DNA polymerase.SELECTED DRAWING: None 【課題】増大したプライマー及び／又は鋳型識別活性を有する突然変異体ＤＮＡポリメラーゼ、並びに遺伝子診断分析用のポリメラーゼに基づくアッセイのための方法を提供する。【解決手段】未修飾ＴａｑＤＮＡポリメラーゼと比較して増大した鋳型識別活性を有する突然変異体ＴａｑＤＮＡポリメラーゼであって、突然変異体ＴａｑＤＮＡポリメラーゼのアミノ酸配列が、未修飾ＴａｑＤＮＡポリメラーゼの特定の残基位置の置換を含む、突然変異体ＴａｑＤＮＡポリメラーゼを提供する。【選択図】なし