COMPOSITION FOR NUCLEIC ACID AMPLIFICATION

To provide a high-speed and high-sensitivity PCR method in real-time PCR targeting virus RNA gene.SOLUTION: A composition for nucleic acid amplification contains following components: (A) DNA polymerase, (B) reverse transcriptase 1.5-4.5 U/μL, (C) forward primer 1.2 μM or more and (D) reverse primer...

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Bibliographische Detailangaben
Hauptverfasser:	NAGAI SHUSUKE, TAKASHIMA MIZUKI, FURUYA SHUNSUKE, TAKEUCHI RYOTA
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a high-speed and high-sensitivity PCR method in real-time PCR targeting virus RNA gene.SOLUTION: A composition for nucleic acid amplification contains following components: (A) DNA polymerase, (B) reverse transcriptase 1.5-4.5 U/μL, (C) forward primer 1.2 μM or more and (D) reverse primer 1.2 μM or more.SELECTED DRAWING: Figure 2 【課題】ウイルスＲＮＡ遺伝子を標的とするリアルタイムＰＣＲにおける高速で感度の良いＰＣＲ方法の提供。【解決手段】以下の成分を含有する核酸増幅用組成物。（Ａ）ＤＮＡポリメラーゼ（Ｂ）逆転写酵素１．５〜４．５Ｕ／μＬ（Ｃ）フォワードプライマー１．２μＭ以上（Ｄ）リバースプライマー１．２μＭ以上【選択図】図２