OLIGONUCLEOTIDE SET FOR DETERMINING DNA TYPE OF CUTIBACTERIUM ACNES, KIT, AND DETERMINATION METHOD OF DNA TYPE OF CUTIBACTERIUM ACNES

To establish a test system that can further accurately, quickly, and easily identify the DNA type of Cutibacterium acnes.SOLUTION: There are provided: an oligonucleotide set for determining the DNA type of Cutibacterium acnes by a nucleic acid amplification method that targets a partial region of ea...

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Hauptverfasser: TAKASAKI KAZUTO, NAKADA SATORU, MORINAKA RIEKO, MIURA SHIHORI, AKAZA MASAFUMI
Format: Patent
Sprache:eng ; jpn
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Zusammenfassung:To establish a test system that can further accurately, quickly, and easily identify the DNA type of Cutibacterium acnes.SOLUTION: There are provided: an oligonucleotide set for determining the DNA type of Cutibacterium acnes by a nucleic acid amplification method that targets a partial region of each gene of JCM18918_744 gene specific for D1 type, TIIST44_06360 gene specific for D2 type, and FB33_0108 gene specific for D3 type, of Cutibacterium acnes; a kit; and a determination method of the DNA type of Cutibacterium acnes, which comprises the steps of performing an amplification reaction by a nucleic acid amplification method using the oligonucleotide set, and detecting an amplified product.SELECTED DRAWING: Figure 1 【課題】アクネ菌(Cutibacterium acnes)のDNAタイプをより正確、迅速、かつ簡便に判別できる検査系を確立すること。【解決手段】アクネ菌(Cutibacterium acnes)のD1タイプに特異的なJCM18918_744遺伝子、D2タイプに特異的なTIIST44_06360遺伝子、D3タイプに特異的なFB33_0108遺伝子の各遺伝子の部分領域を標的として核酸増幅法によってアクネ菌のDNAタイプを判別するためのオリゴヌクレオチドセット、キット、及び当該オリゴヌクレオチドセットを用いて核酸増幅法によって増幅反応を行う工程と、増幅産物を検出する工程を含む、アクネ菌のDNAタイプの判別方法。【選択図】図1