OLIGONUCLEOTIDE SET FOR DETECTION OF INDIGENOUS BACTERIUM IN SKIN, KIT, AND METHOD FOR DETECTING INDIGENOUS BACTERIUM IN SKIN

To establish test systems that can accurately, quickly, easily, and simultaneously detect indigenous bacterium in the skin of the genera Cutibacterium, Staphylococcus, and Malassezia.SOLUTION: The present invention relates to: an oligonucleotide set for individually and simultaneously detecting indigenous bacterium in the skin belonging to the following three genera by a nucleic acid amplification method by targeting a partial region of each gene of FB33_0681 gene or FB33_0824 gene specific for indigenous bacterium in the skin of the genus Cutibacterium, SAOUHSC_01002 gene specific for indigenous bacterium in the skin of the genus Staphylococcus, and MGL_1553 gene specific for indigenous bacterium in the skin of the genus Malassezia; a kit; and a method for detecting indigenous bacterium in the skin which comprises the processes of: performing an amplification reaction by the nucleic acid amplification method using the oligonucleotide set; and detecting amplification products.SELECTED DRAWING: Figure 1 【課題】キューティバクテリウム（Cutibacterium）属、スタフィロコッカス（Staphylococcus）属、及びマラセチア（Malassezia）属の皮膚常在菌を正確、迅速、かつ簡便に、しかも同時に検出できる検査系を確立すること。【解決手段】キューティバクテリウム（Cutibacterium）属の皮膚常在菌に特異的なFB33_0681遺伝子又はFB33_0824遺伝子、スタフィロコッカス（Staphylococcus）属の皮膚常在菌に特異的なSAOUHSC_01002遺伝子、マラセチア（Malassezia）属の皮膚常在菌に特異的なMGL_1553遺伝子の各遺伝子の部分領域を標的として核酸増幅法によって上記の３つの属に属する皮膚常在菌を個々に、また、同時に検出するためのオリゴヌクレオチドセット、キット、及び当該オリゴヌクレオチドセットを用いて核酸増幅法によって増幅反応を行う工程と、増幅産物を検出する工程を含む、皮膚常在菌の検出方法。【選択図】図１