CELL CULTURE APPARATUS AND CULTURE MEDIUM EXCHANGE METHOD

To protect cells during discharge of medium from a culture vessel in a cell culture device.SOLUTION: A centrally crowded state 83 of a cell population is formed by subjecting a culture container to a shaking motion prior to removing the culture medium by suction. In the centrally crowded state 83, c...

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Hauptverfasser:	HONGO AKANE, MAKITA NAOHIRO, MINAMI KAZUNARI, KATOU MIDORI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To protect cells during discharge of medium from a culture vessel in a cell culture device.SOLUTION: A centrally crowded state 83 of a cell population is formed by subjecting a culture container to a shaking motion prior to removing the culture medium by suction. In the centrally crowded state 83, cell clusters 84 are separated from a discharge port 58a. After removal of the culture medium by suction, a new culture medium is introduced into the culture container. After introduction of the new culture medium, a generally dispersed state of the cell population is formed.SELECTED DRAWING: Figure 12 【課題】細胞培養装置において、培養容器からの培地の排出の際に細胞を保護する。【解決手段】培地の吸引除去に先立って、培養容器を揺動運動させることにより、細胞集団の中央密集状態８３が形成される。中央密集状態８３では、細胞密集体８４は、排出口５８ａから隔てられている。培地の吸引除去の後、培養容器内に新しい培地が導入される。新しい培地の導入後、細胞集団の全体分散状態が形成される。【選択図】図１２