BIOSENSOR FOR MEASURING GLUCOSE

To provide a biosensor for measuring glucose using FAD-conjugated glucose dehydrogenase.SOLUTION: A biosensor for measuring glucose comprises an enzyme reaction layer arranged on an electrode system, in which the enzyme reaction layer includes recombinant polypeptide derived from Aspergillus oryzae...

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Hauptverfasser:	YADA TAKAKO, HONDA MICHINARI, MIYAMOTO HIROSHI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a biosensor for measuring glucose using FAD-conjugated glucose dehydrogenase.SOLUTION: A biosensor for measuring glucose comprises an enzyme reaction layer arranged on an electrode system, in which the enzyme reaction layer includes recombinant polypeptide derived from Aspergillus oryzae strain which has FAD-conjugated glucose dehydrogenase activity and electron receptor, and the biosensor has excellent reactivity, thermal stability, and substrate-recognition property to glucose and low activity to maltose.SELECTED DRAWING: Figure 1 【課題】ＦＡＤ結合型グルコース脱水素酵素を用いたグルコース測定用バイオセンサの提供。【解決手段】電極系上に配置された酵素反応層を備え、該酵素反応層はＦＡＤ結合型グルコース脱水素酵素活性を有するアスペルギルス・オリゼ株由来の組換型ポリペプチド及び電子受容体を含むグルコース測定用バイオセンサであって、グルコースに対する反応性、熱安定性、基質認識性に優れ、マルトースに対する作用性が低いグルコース測定用バイオセンサ。【選択図】図１