METHOD FOR EVALUATING EFFECT ON SKIN BARRIER FUNCTION

To provide a method capable of evaluating the effect of an article or substance in contact with skin on a skin barrier function in a non-invasive manner, safely with little burden on a subject, simply and in a short time.SOLUTION: A method of evaluating the effect of an article or substance in contact with skin on a skin barrier function includes the steps of: (a) bringing a test article or substance into contact with human skin; (b) measuring a molecular association state of intercellular lipids of the human skin after release of the contact; and (c) comparing the molecular association state of the intercellular lipids with a reference value, and evaluating the effect of the test article or substance on the skin barrier function.SELECTED DRAWING: None 【課題】非侵襲的で、被験者に対して負担が少なく安全、簡便かつ短時間に、皮膚に接触する物品又は物質が皮膚バリア機能に与える影響を評価し得る方法を提供する。【解決手段】以下の工程（ａ）〜（ｃ）を含む、皮膚に接触する物品又は物質の皮膚バリア機能に与える影響の評価方法；（ａ）被験物品又は物質をヒト皮膚に接触させる工程、（ｂ）前記接触解除後に当該ヒト皮膚の細胞間脂質の分子会合状態を測定する工程、（ｃ）前記細胞間脂質の分子会合状態を基準値と比較し、前記被験物品又は物質が、皮膚バリア機能に与える影響を評価する工程。【選択図】なし