METHOD FOR BIOCATALYTIC CYCLIZATION OF TERPENES, AND CYCLASE MUTANTS WHICH CAN BE USED IN THAT METHOD

To provide new types of mutants with cyclase activity, and a method for biocatalytic cyclization of terpenes.SOLUTION: The invention relates to: enzyme mutants including specific amino acid sequences and having cyclase activity for catalyzing the cyclization of a citronellal isomer into an isopulego...

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Bibliographische Detailangaben
Hauptverfasser:	SILVIA RACOLTA, DIETER JENDROSSEK, HAUER BERNHARD, JUERGEN PLEISS, GABRIELE SIEDENBURG, DEMET SIRIM, MICHAEL BREUER
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide new types of mutants with cyclase activity, and a method for biocatalytic cyclization of terpenes.SOLUTION: The invention relates to: enzyme mutants including specific amino acid sequences and having cyclase activity for catalyzing the cyclization of a citronellal isomer into an isopulegol isomer; a method for producing menthol or isopulegol by the cyclization of citronellal using the enzyme mutants; and a method for biocatalytic conversion of other compounds with terpene type structural motifs using the enzyme mutants.SELECTED DRAWING: None 【課題】シクラーゼ活性を有する新しい型の変異体、及びテルペンの生体触媒環化のための方法の提供。【解決手段】特定のアミノ酸配列を含み、シトロネラール異性体からイソプレゴール異性体への環化を触媒するシクラーゼ活性を有する酵素変異体、前記酵素変異体を用いてシトロネラールの環化によってイソプレゴールまたはメントールを生成するための方法、及び、前記酵素変異体を用いてテルペン型構造モチーフを有する他の化合物を生体触媒変換する方法。【選択図】なし