METHODS AND SYSTEMS FOR CONVERTING PRECURSOR CELLS INTO GASTRIC TISSUES THROUGH DIRECTED DIFFERENTIATION

To provide methods for promoting definitive endoderm formation from human pluripotent stem cells, and methods for promoting gastric organoid or gastric tissue formations from differentiated definitive endoderm.SOLUTION: A method of inducing formation of a gastric tissue comprises the steps of: a) ac...

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Hauptverfasser:	KYLE WILLIAM MCCRACKEN, JAMES MACORMACK WELLS
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide methods for promoting definitive endoderm formation from human pluripotent stem cells, and methods for promoting gastric organoid or gastric tissue formations from differentiated definitive endoderm.SOLUTION: A method of inducing formation of a gastric tissue comprises the steps of: a) activating one or more signaling pathways within a precursor cell, where the one or more signaling pathways are selected from among a WNT signaling pathway, a WNT/FGF signaling pathway, a FGF signaling pathway, and a combination thereof; b) inhibiting a BMP signaling pathway within the precursor cell; and c) administering retinoic acid to the precursor cell.SELECTED DRAWING: None 【課題】ヒト多能性幹細胞からの胚体内胚葉形成を促進するための方法と、分化した胚体内胚葉からの胃オルガノイド又は胃組織形成を促進するための方法の提供。【解決手段】ａ）前駆細胞内の１つ以上のシグナル伝達経路を活性化するステップであって、前記１つ以上のシグナル伝達経路が、ＷＮＴシグナル伝達経路、ＷＮＴ／ＦＧＦシグナル伝達経路、ＦＧＦシグナル伝達経路、又はそれらの組み合わせから選択されるステップと、ｂ）前記前駆細胞内のＢＭＰシグナル伝達経路を阻害するステップと、ｃ）前記前駆細胞にレチノイン酸を投与するステップとを含む、胃組織の形成を誘導する方法。【選択図】なし