POLYPEPTIDES COMPRISING MODIFIED BACTERIOPHAGE g3p AMINO ACID SEQUENCE LACKING GLYCOSYLATION SIGNAL

To provide polypeptides comprising a modified bacteriophage g3p amino acid sequence lacking a glycosylation signal.SOLUTION: The invention relates to polypeptides that comprise a portion of filamentous bacteriophage gene 3 protein (g3p) sufficient to bind to and/or disaggregate amyloid, e.g., the N1...

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Hauptverfasser:	PROSCHITSKY MING, RAJARAMAN KRISHNAN, RICHARD FISHER, ROBERT G E HOLGATE, EVA ASP, TIMOTHY D JONES, FRANCIS J CARR
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide polypeptides comprising a modified bacteriophage g3p amino acid sequence lacking a glycosylation signal.SOLUTION: The invention relates to polypeptides that comprise a portion of filamentous bacteriophage gene 3 protein (g3p) sufficient to bind to and/or disaggregate amyloid, e.g., the N1-N2 portion of g3p and mutants and fragments thereof, where that g3p amino acid sequence has been modified through amino acid deletion, insertion or substitution to remove a putative glycosylation signal. The invention further relates to such polypeptides that are also modified through additional amino acid substitution to be substantially less immunogenic than the corresponding wild-type g3p amino acid sequence when used in vivo. The polypeptides of the invention retain their ability to bind and/or disaggregate amyloid.SELECTED DRAWING: None 【課題】グリコシル化シグナルを欠く改変バクテリオファージｇ３ｐアミノ酸配列を含むポリペプチドの提供。【解決手段】本発明は、アミロイドに結合するおよび／またはアミロイドを脱凝集させるのに十分な、糸状バクテリオファージ遺伝子３タンパク質（ｇ３ｐ）の一部分、例えば、ｇ３ｐのＮ１−Ｎ２部分ならびにその変異体および断片、を含むポリペプチドに関し、ここで、そのｇ３ｐアミノ酸配列は、推定グリコシル化シグナルを除去するように、アミノ酸欠失、挿入または置換を介して改変されている。本発明は、ｉｎｖｉｖｏで使用される場合に、対応する野生型ｇ３ｐアミノ酸配列よりも免疫原性が実質的に少なくなるように、さらなるアミノ酸置換を介しても改変された、かかるポリペプチドにさらに関する。本発明のポリペプチドは、アミロイドに結合するおよび／または脱凝集させるそれらの能力を保持する。【選択図】なし