PCR METHOD

To prevent contamination of a PCR reaction vessel.SOLUTION: A PCR reaction vessel 10 comprises: a substrate 14; a flow channel 12 formed on the substrate 14; a pair of first filter 28 and second filter 30 provided on both ends of the flow channel 12; a pair of first air communication port 24 and sec...

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Hauptverfasser: NAGAI SHUSUKE, NISHIZAWA NAOFUMI, FUKUZAWA TAKASHI
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creator NAGAI SHUSUKE
NISHIZAWA NAOFUMI
FUKUZAWA TAKASHI
description To prevent contamination of a PCR reaction vessel.SOLUTION: A PCR reaction vessel 10 comprises: a substrate 14; a flow channel 12 formed on the substrate 14; a pair of first filter 28 and second filter 30 provided on both ends of the flow channel 12; a pair of first air communication port 24 and second air communication port 26 which communicate with the flow channel 12 through the first filter 28 and the second filter 30; a thermal cycle region 12e formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch point 112c formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch flow channel 131 whose one end is connected to the branch point 112c; and a sample introduction port 133 formed on the other end of the branch flow channel 131.SELECTED DRAWING: Figure 1 【課題】PCR反応容器のコンタミネーションを防止する。【解決手段】PCR反応容器10は、基板14と、基板14に形成された流路12と、流路12の両端に設けられた一対の第1フィルタ28および第2フィルタ30と、第1フィルタ28および第2フィルタ30を通じて流路12と連通する一対の第1空気連通口24および第2空気連通口26と、流路12における第1フィルタ28および第2フィルタ30の間に形成されたサーマルサイクル領域12eと、流路12における第1フィルタ28および第2フィルタ30の間に形成された分岐点112cと、一端が分岐点112cに接続された分岐流路131と、分岐流路131の他端に形成された試料導入口133とを備える。【選択図】図1
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a pair of first air communication port 24 and second air communication port 26 which communicate with the flow channel 12 through the first filter 28 and the second filter 30; a thermal cycle region 12e formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch point 112c formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch flow channel 131 whose one end is connected to the branch point 112c; and a sample introduction port 133 formed on the other end of the branch flow channel 131.SELECTED DRAWING: Figure 1 【課題】PCR反応容器のコンタミネーションを防止する。【解決手段】PCR反応容器10は、基板14と、基板14に形成された流路12と、流路12の両端に設けられた一対の第1フィルタ28および第2フィルタ30と、第1フィルタ28および第2フィルタ30を通じて流路12と連通する一対の第1空気連通口24および第2空気連通口26と、流路12における第1フィルタ28および第2フィルタ30の間に形成されたサーマルサイクル領域12eと、流路12における第1フィルタ28および第2フィルタ30の間に形成された分岐点112cと、一端が分岐点112cに接続された分岐流路131と、分岐流路131の他端に形成された試料導入口133とを備える。【選択図】図1</description><language>eng ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; 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NISHIZAWA NAOFUMI ; FUKUZAWA TAKASHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2020022520A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NAGAI SHUSUKE</creatorcontrib><creatorcontrib>NISHIZAWA NAOFUMI</creatorcontrib><creatorcontrib>FUKUZAWA TAKASHI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NAGAI SHUSUKE</au><au>NISHIZAWA NAOFUMI</au><au>FUKUZAWA TAKASHI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PCR METHOD</title><date>2020-02-13</date><risdate>2020</risdate><abstract>To prevent contamination of a PCR reaction vessel.SOLUTION: A PCR reaction vessel 10 comprises: a substrate 14; 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title PCR METHOD
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