PCR METHOD

To prevent contamination of a PCR reaction vessel.SOLUTION: A PCR reaction vessel 10 comprises: a substrate 14; a flow channel 12 formed on the substrate 14; a pair of first filter 28 and second filter 30 provided on both ends of the flow channel 12; a pair of first air communication port 24 and second air communication port 26 which communicate with the flow channel 12 through the first filter 28 and the second filter 30; a thermal cycle region 12e formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch point 112c formed between the first filter 28 and the second filter 30 in the flow channel 12; a branch flow channel 131 whose one end is connected to the branch point 112c; and a sample introduction port 133 formed on the other end of the branch flow channel 131.SELECTED DRAWING: Figure 1 【課題】ＰＣＲ反応容器のコンタミネーションを防止する。【解決手段】ＰＣＲ反応容器１０は、基板１４と、基板１４に形成された流路１２と、流路１２の両端に設けられた一対の第１フィルタ２８および第２フィルタ３０と、第１フィルタ２８および第２フィルタ３０を通じて流路１２と連通する一対の第１空気連通口２４および第２空気連通口２６と、流路１２における第１フィルタ２８および第２フィルタ３０の間に形成されたサーマルサイクル領域１２ｅと、流路１２における第１フィルタ２８および第２フィルタ３０の間に形成された分岐点１１２ｃと、一端が分岐点１１２ｃに接続された分岐流路１３１と、分岐流路１３１の他端に形成された試料導入口１３３とを備える。【選択図】図１