BIOGENIC SUBSTANCE MEASUREMENT KIT AND METHOD IN WHICH MEASUREMENT SENSITIVITY IS IMPROVED

To provide a measurement kit and measurement method of a biogenic substance capable of achieving stable measurement accuracy without reducing sensitivity, in measurement of a biogenic substance which is performed using color reaction generated by oxidation condensation of 4-amino antipyrine and hydrogen donator in coexistence of hydrogen peroxide generated by making an oxidation enzyme act to a biogenic substance and peroxidase.SOLUTION: There are provided a measurement kit and a measurement method of biogenic substance, in the measurement kit of biogenic substance, hydrogen peroxide generated by making an oxidation enzyme act to a biogenic substance performs oxidation condensation of 4-amino antipyrine and hydrogen donator in coexistence of peroxidase, therefore color reaction of a reaction liquid is generated, the color reaction is subjected to colorimetric determination. In the measurement kit and measurement method, a density of 4-hydroxy antipyrine in the reaction liquid is equal to or smaller than 1.5 μg/ml.SELECTED DRAWING: None 【課題】 ４−アミノアンチピリンが、生体成分に酸化酵素を作用させ生成した過酸化水素とペルオキシダーゼの共存下で、水素供与体と酸化縮合して生じた呈色反応を利用して行う生体成分の測定において、感度低下を生じさせることなく、安定した測定精度を示す生体成分の測定キットや測定方法を提供する。【解決手段】 生体成分に酸化酵素を作用させ生成した過酸化水素が、ペルオキシダーゼの共存下、４−アミノアンチピリンと水素供与体を酸化縮合することにより生じた反応液の呈色を比色定量する生体成分測定キットであって、前記反応液中の４−ヒドロキシアンチピリンの濃度が１．５μｇ／ｍｌ以下であることを特徴とする生体成分測定キット及び生体成分測定方法。【選択図】 なし