PROCESSES FOR PRODUCING POXVIRUS USING PROLIFERATIVE HELPER VACCINIA VIRUS

To provide efficient and safe processes for producing poxvirus.SOLUTION: Disclosed herein is a process for producing poxvirus comprising the steps of: infecting a cultured cell with a proliferative helper vaccinia virus and culturing the cell, and transfecting the cultured cell with DNA of a virus o...

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Bibliographische Detailangaben
Hauptverfasser:	OKITA TAKESHI, KURIYAMA SACHIKO, SAKAGUCHI MASASHI, SUENAGA KIYOTAKA
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide efficient and safe processes for producing poxvirus.SOLUTION: Disclosed herein is a process for producing poxvirus comprising the steps of: infecting a cultured cell with a proliferative helper vaccinia virus and culturing the cell, and transfecting the cultured cell with DNA of a virus of interest and culturing the transfected cell, and harvesting the virus of interest from the cultured cell.SELECTED DRAWING: None 【課題】ポックスウイルスの効率的で安全な製造方法を提供する。【解決手段】ポックスウイルスの製造方法であって、培養細胞にヘルパーウイルスとして増殖性のヘルパーワクシニアウイルスを感染させ培養する工程と、前記培養細胞に目的ウイルスＤＮＡをトランスフェクションし培養する工程と、前記培養細胞から目的ウイルスを回収する工程と、を有する方法。【選択図】なし