RECOMBINASE POLYMERASE AMPLIFICATION

To provide recombinase polymerase amplification.SOLUTION: The present invention provides novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also provides di...

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Hauptverfasser:	PIEPENBURG OLAF, PARKER MATHEW JAMES DAVID, ARMES NIALL ANTONY
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide recombinase polymerase amplification.SOLUTION: The present invention provides novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also provides different recombinase "systems" having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed. More specifically, the present invention relates to the use of T6, Rb69, Aeh1, and KVP40 hybrid and engineered proteins, and the use of such proteins in recombinase polymerase amplification assays.SELECTED DRAWING: None 【課題】レコンビナーゼポリメラーゼ増幅を提供すること。【解決手段】本発明は、新規の、多様な、ハイブリッドおよび作成されたレコンビナーゼ酵素、ならびにＤＮＡ増幅アッセイを行うための、関連組換え因子をともなうそのような蛋白質の使用を、その特色とする。また、本発明は、ＤＮＡ増幅アッセイにおける異なる生化学的活性を有する異なるレコンビナーゼ「系」、ならびに負荷因子、一本鎖ＤＮＡ結合蛋白質（ＳＳＢ）、および使用される密集剤の量についての異なる要求も、その特色とする。さらに詳しくは、本発明は、Ｔ６、Ｒｂ６９、Ａｅｈｌ、およびＫＶＰ４０ハイブリッドおよび作成蛋白質の使用、およびそのような蛋白質のレコンビナーゼポリメラーゼ増幅アッセイにおける使用に関する。【選択図】なし