IMPROVED NITRILE HYDRATASE

To provide a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures, and a method for producing the nitrile hydratase.SOLUTION: A nitrile hydratase has at least one amino acid mutation selected from among (a) to (d) in the following, in the amino acid se...

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Bibliographische Detailangaben
Hauptverfasser:	KITAHARA AYA, WATANABE FUMIAKI, WAKAMATSU YOSHINORI, AKIYAMA TAKAMICHI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures, and a method for producing the nitrile hydratase.SOLUTION: A nitrile hydratase has at least one amino acid mutation selected from among (a) to (d) in the following, in the amino acid sequence in an α subunit of the nitrile hydratase (Xto Xrepresent independent arbitrary amino acid residues): (a) Xbeing valine or glycine, (b) Xbeing valine or threonine, (c) Xbeing an amino acid selected from the group consisting of isoleucine, leucine, methionine and threonine, and (d) Xbeing leucine.SELECTED DRAWING: None 【課題】高温下でのアミド化合物耐性が向上した新規な改良型ニトリルヒドラターゼ、及び該ニトリルヒドラターゼの製造方法の提供。【解決手段】該ニトリルヒドラターゼαサブユニットのアミノ酸配列において、下記（ａ）〜（ｄ）から選ばれる少なくとも一つのアミノ酸変異を有するニトリルヒドラターゼ（Ｘ１〜Ｘ２７は独立した任意のアミノ酸残基を表す）。（ａ）Ｘ１がバリン又はグリシン、（ｂ）Ｘ９がバリン又はトレオニン、（ｃ）Ｘ２３がイソロイシン、ロイシン、メチオニン、及びトレオニンよりなる群から選択されるアミノ酸、（ｄ）Ｘ２４がロイシン【選択図】なし