UV OZONE-PLASMA COMPOSITE TREATMENT METHOD AND TREATMENT APPARATUS

To provide a surface modification method for culturing an adherent cell which is suitable for cell adhesion even more.SOLUTION: The present invention is a method for manufacturing a cell-carrying base material having a cell-carrying face which has non fluorine-based resin as a main component, in whi...

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Bibliographische Detailangaben
Hauptverfasser:	MIYATA SHOGO, OHIRA MICHIO, NAKADA HIDEO, TAKAHASHI SHUICHI, FUJITA ATSUSHI, FUKUDA KEIICHI, SUZUKI HAYATO, TOYAMA SHUGO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY GENERAL PROCESSES OF COMPOUNDING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE WORKING-UP
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Beschreibung
Zusammenfassung:	To provide a surface modification method for culturing an adherent cell which is suitable for cell adhesion even more.SOLUTION: The present invention is a method for manufacturing a cell-carrying base material having a cell-carrying face which has non fluorine-based resin as a main component, in which the method includes: a UV irradiation process of irradiating a cell-carrying face of the base material with UV under humidifying environment in an oxygen and/or ozone feeding atmosphere; and a plasma irradiation process of irradiating the cell-carrying face of the base material with plasma, and a device to be used for the method is provided. The present invention provides a culture method for culturing a cell on the cell-carrying face of a cell-carrying base material which is obtained by the manufacturing method.SELECTED DRAWING: Figure 11 【課題】本発明は，より細胞接着に適した接着性細胞を培養するための表面修飾方法を提供することを目的とする。【解決手段】本発明は細胞担持面を有する非フッ素系樹脂を主成分とする細胞担持用基材の製造方法であって，加湿環境下，酸素及び／又はオゾン供給雰囲気中で，前記基材の細胞担持面にＵＶを照射するＵＶ照射工程，及び前記基材の細胞担持面にプラズマを照射するプラズマ照射工程を含む方法，及び当該方法に用いるための装置を提供する。また，本発明は，前記製造方法により得られた細胞担持用基材の細胞担持面上で細胞を培養することを含む培養方法を提供する。【選択図】図１１