METHODS FOR LONG TERM STORAGE OF NUCLEIC ACID IN BIOLOGICAL SAMPLES

To provide methods for preserving nucleic acids of cells, bacteria or viruses contained in biological samples stably for a long term, where the analyte using the methods can supply to the concentration and culture steps of the cells, bacteria or viruses.SOLUTION: To solve the problem, the invention...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	KITAMORI YUKA, SAITO TOSHIKAZU
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	To provide methods for preserving nucleic acids of cells, bacteria or viruses contained in biological samples stably for a long term, where the analyte using the methods can supply to the concentration and culture steps of the cells, bacteria or viruses.SOLUTION: To solve the problem, the invention provides a method for preserving a nucleic acid contained in a cell, bacterium or virus within a biological sample, the method comprising a step of mixing the biological sample and a reducing agent, where the reducing agent is one or more selected from the group consisting of dithiothreitol, dithioerythritol, mercaptoethanol, tris (2-carboxyethyl)phosphine hydrochloride (TCEP-HCl), N-acetyl-L-cysteine (NALC), and L-cysteine hydrochloride.SELECTED DRAWING: None 【課題】生体試料中の細胞、細菌またはウィルス等の中に含まれる核酸を長期間安定的に保存するための方法を提供すること。上記方法を用いた検体は、細胞、細菌またはウィルス等を濃縮する工程、培養工程に供することが可能であること。【解決手段】生体試料中の細胞、細菌またはウィルス等の中に含まれる核酸を保存する方法であって、前記生体試料と還元剤を混合する工程を含み、前記還元剤が、ジチオスレイトール、ジチオエリトリトール、メルカプトエタノール、トリス（２−カルボキシエチル）フォスフィンハイドロクロライド（ＴＣＥＰ−ＨＣｌ）、Ｎ−アセチル−Ｌ−システイン（ＮＡＬＣ）、およびＬ−システインハイドロクロライドからなる群より選ばれる１種以上を含む、核酸の保存方法により、前記課題を解決する。【選択図】なし