METHOD OF BIOCATALYTIC CYCLIZATION OF TERPENES AND CYCLASE VARIANTS OBTAINED THEREBY

PROBLEM TO BE SOLVED: To provide an alternative method to publicly-known chemical cyclization methods for terpenes that cyclize terpene compounds with an enzymatic catalyst, and a novel biocatalytic catalyst to be used for cyclizing terpenes.SOLUTION: An enzymatic mutant having cyclase activity sele...

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Hauptverfasser:	SILVIA RACOLTA, DIETER JENDROSSEK, HAUER BERNHARD, JUERGEN PLEISS, GABRIELE SIEDENBURG, DEMET SIRIM, MICHAEL BREUER
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide an alternative method to publicly-known chemical cyclization methods for terpenes that cyclize terpene compounds with an enzymatic catalyst, and a novel biocatalytic catalyst to be used for cyclizing terpenes.SOLUTION: An enzymatic mutant having cyclase activity selected from variants of a wild type enzyme comprising an amino acid sequence selected from a specific 325 amino acid sequence or a partial sequence thereof where said enzymatic variant catalyzes at least cyclization from the citronellal isomer to at least one isopulegol isomer.SELECTED DRAWING: None 【課題】テルペン化合物を酵素触媒によって環化させるテルペンのための公知の化学的環化方法の代替法およびテルペンの環化に使用され得る新規生体触媒の提供。【解決手段】特定の３２５種のアミノ酸配列又はその部分配列から選択されるアミノ酸配列を含む、野生型酵素の変異体から選択される、シクラーゼ活性を有する酵素変異体であって、シトロネラール異性体から、少なくとも一つのイソプレゴール異性体への少なくとも環化を触媒する、前記酵素変異体。【選択図】なし