VARIANT TYROSINASES AND USES THEREOF

PROBLEM TO BE SOLVED: To provide methods for producing betalain pigment etc., using microorganisms with improved productivity.SOLUTION: Disclosed herein is a protein with a tyrosinase activity, which comprises a specific amino acid sequence or an amino acid sequence having 90% or more identity to th...

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Hauptverfasser:	MAEDA TAKAYUKI, YAMAMOTO TAKASHI, MIYAOKU KOHEI, YUMURA SHUICHI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF COMPOUNDS OF UNKNOWN CONSTITUTION CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide methods for producing betalain pigment etc., using microorganisms with improved productivity.SOLUTION: Disclosed herein is a protein with a tyrosinase activity, which comprises a specific amino acid sequence or an amino acid sequence having 90% or more identity to the specific amino acid sequence but with the following variations of (i) and/or (ii) in which: (i) amino acid residues corresponding to one or more amino acid residues selected from the group consisting of S2, N3, Y5, I28, K30, G46, F48, S54, N57, W68, E71, S104, D127, N144, D166, D170, K173, W182, E187, N188, S189, Q193, E195, T220, H230, N233, W241, I243, Q251, N261, D264, Y267, P273, G283, and I288 in the specific amino acid sequence are substituted; and/or (ii) the amino acid residue corresponding to V218 of the specific amino acid sequence is substituted.SELECTED DRAWING: None 【課題】微生物を用いてベタレイン色素等の製造方法の生産性を向上させる。【解決手段】特定のアミノ酸配列、又は特定のアミノ酸配列と90％以上の同一性を有するアミノ酸配列を有し、かつ、下記（ｉ）及び／又は（ｉｉ）の変異を有する、チロシナーゼ活性を有するタンパク質。（ｉ）特定のアミノ酸配列におけるS2、N3、Y5、I28、K30、G46、F48、S54、N57、W68、E71、S104、D127、N144、D166、D170、K173、W182、E187、N188、S189、Q193、E195、T220、H230、N233、W241、I243、Q251、N261、D264、Y267、P273、G283、及びI288からなる群より選ばれる１又は複数のアミノ酸残基に相当するアミノ酸残基が、置換された変異。（ｉｉ）特定のアミノ酸配列におけるV218に相当するアミノ酸残基が置換された変異。【選択図】なし