IMMUNO-BASED BOTULINUM TOXIN SEROTYPE-A ACTIVITY ASSAYS

PROBLEM TO BE SOLVED: To provide immuno-based botulinum toxin serotype-A activity assays.SOLUTION: A method of detecting botulinum neurotoxin serotype A (BoNT/A) activity in a sample comprises: a. bringing a test sample suspected of comprising BoNT/A into contact with a cell from an established cell...

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Hauptverfasser:	HODGES D DIANNE, FERNANDEZ-SALAS ESTER, WANG JOANNE, GARAY PATTON E, WONG LINA S, AOKI K ROGER
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide immuno-based botulinum toxin serotype-A activity assays.SOLUTION: A method of detecting botulinum neurotoxin serotype A (BoNT/A) activity in a sample comprises: a. bringing a test sample suspected of comprising BoNT/A into contact with a cell from an established cell line expressing a SNAP-25 polypeptide consisting essentially of at least a portion of human SNAP-25 comprising a sequence cleavable by BoNT/A; b. isolating a SNAP-25 cleavage product from the cell; and c. bringing the SNAP-25 cleavage product into contact with an anti-SNAP-25 antibody linked to a solid phase support.SELECTED DRAWING: None 【課題】免疫基盤ボツリヌス毒素血清Ａ型活性アッセイを提供する。【解決手段】ａ．ＢｏＮＴ／Ａにより切断可能である配列を含むヒトＳＮＡＰ−２５の少なくとも一部から本質的になるＳＮＡＰ−２５ポリペプチドを発現する確立された細胞株からの細胞を、ＢｏＮＴ／Ａを含むことが疑われる被験試料と接触させること、ｂ．該細胞からＳＮＡＰ−２５切断生成物を単離すること；ｃ．ＳＮＡＰ−２５切断生成物を固相支持体に連結された抗ＳＮＡＰ−２５抗体と接触させること、を含む、試料中のボツリヌス神経毒血清Ａ型（ＢｏＮＴ／Ａ）活性を検出する方法。【選択図】なし