AMYLASE ACTIVITY MEASUREMENT REAGENT AND AMYLASE ACTIVITY MEASUREMENT METHOD

PROBLEM TO BE SOLVED: To provide an α-amylase activity measurement reagent and an α-amylase activity measurement method that avoid bad influence on reactivity in JCCLS-SOP method.SOLUTION: An AMY activity measurement reagent contains ENM as a substrate and α-glycosidase as a coupling enzyme. The rea...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	NISHIYAMA KYOKO, YASUDA YUKIO, YAMAMOTO YUKITOSHI, TAKEGAWA YUKO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide an α-amylase activity measurement reagent and an α-amylase activity measurement method that avoid bad influence on reactivity in JCCLS-SOP method.SOLUTION: An AMY activity measurement reagent contains ENM as a substrate and α-glycosidase as a coupling enzyme. The reagent contains a nonionic surfactant of a structure represented by formula (1). The reagent is to measure AMY activity in a sample. (R1 is a C4-12 linear/branched alkyl group; R2-R5 are independently represent H, C, or a 1-4 linear/branched alkyl group; X is an integer of 20-45).SELECTED DRAWING: None 【課題】ＪＣＣＬＳ-ＳＯＰ法との反応性に影響を生じないα−アミラーゼ活性測定試薬及びα−アミラーゼ活性の測定方法を提供する。【解決手段】ＥＮＭを基質として、α−グルコシターゼを共役酵素として含む、ＡＭＹ活性測定試薬において、式（１）で表される構造の非イオン性界面活性剤を含有する、試料中のＡＭＹ活性測定試薬。（Ｒ１はＣ４〜１２の直鎖状／分岐状のアルキル基；Ｒ２〜Ｒ5はＣ各々独立にＨ、Ｃ；１〜４の直鎖状／分枝状のアルキル基；Ｘは２０〜４５の整数）【選択図】なし