CELL ORGANELLE-LOCALIZED FUSION PROTEIN

PROBLEM TO BE SOLVED: To provide a cell organelle-localized fusion protein that enables high-precision observation of a cell organelle and a cell organelle-localized protein, a nucleic acid encoding the protein, an expression vector containing the nucleic acid, and a cell into which the nucleic acid...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
1. Verfasser:	MORIYA HIROAKI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a cell organelle-localized fusion protein that enables high-precision observation of a cell organelle and a cell organelle-localized protein, a nucleic acid encoding the protein, an expression vector containing the nucleic acid, and a cell into which the nucleic acid has been introduced, and to provide a method for visualizing a cell organelle with a high degree of accuracy.SOLUTION: According to the present invention, there are provided a fusion protein comprising a localization signal peptide to a cell organelle, a fluorescent protein, and a proteasome degradation signal peptide; a nucleic acid encoding the fusion protein; an expression vector containing the nucleic acid; a cell into which the nucleic acid has been introduced; and a method for visualizing a cell organelle, the method comprising the step of expressing the fusion protein in a cell.SELECTED DRAWING: None 【課題】細胞小器官及び細胞小器官局在タンパク質の高精度の観察が可能となる細胞小器官局在性融合タンパク質、該タンパク質をコードする核酸、該核酸を含む発現ベクター、及び該核酸が導入された細胞を提供する。さらに、高精度の細胞小器官を可視化する方法を提供する。【解決手段】細胞小器官への局在化シグナルペプチドと、蛍光タンパク質と、プロテアソーム分解シグナルペプチドとから構成される融合タンパク質、該融合タンパク質をコードする核酸、該核酸を含む発現ベクター、該核酸が導入された細胞、及び該融合タンパク質を細胞内で発現させる工程を含む、細胞小器官を可視化する方法。【選択図】なし