METHOD FOR PREPARING SPHEROID OF PRIMARY CANCER CELL, SPHEROID, SCREENING METHOD, AND DIAGNOSTIC METHOD

PROBLEM TO BE SOLVED: To provide a method for preparing a spheroid by providing, in a tissue contaminated with cells with fast growth rate, a culture environment advantageous for culturing cancer cells while suppressing the proliferation ability of cells having a fast proliferation rate to provide a...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ITO MANABU, NAKATSURA TETSUYA, GOJI HIROSHI, SHIMOMURA MANAMI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for preparing a spheroid by providing, in a tissue contaminated with cells with fast growth rate, a culture environment advantageous for culturing cancer cells while suppressing the proliferation ability of cells having a fast proliferation rate to provide a spheroid containing a cell derived from a primary cancer cell as a major component.SOLUTION: According to the present invention, there is provided a method for preparing a spheroid of a primary cancer cell. The method comprises the steps of: preparing a medium containing at least 1% by volume or more of serum in the total volume; preparing a cell culture substrate that has been subjected to a treatment for suppressing adhesion to cells; sorting cells from a tissue piece containing cancer cells; and seeding the cells on the cell culture substrate in the medium and culturing the cells.SELECTED DRAWING: None 【課題】増殖速度の早い細胞が混入した組織において、増殖速度の早い細胞の増殖能を抑制しつつ、癌細胞の培養に有利な培養環境を提供することにより、初代癌細胞由来の細胞を主要成分として含むスフェロイドが得られる、スフェロイド作製方法を提供すること。【解決手段】初代癌細胞のスフェロイド作製方法であって、全体積中少なくとも１体積％以上の血清を含む培地を用意する工程と、細胞との接着性を抑制する処理を施した細胞培養基材を用意する工程と、癌細胞を含む組織片から細胞を分取する工程と、前記培地中、前記細胞培養基材上に細胞を播種し、前記細胞を培養する工程と、を含むことを特徴とする初代癌細胞のスフェロイド作製方法。【選択図】なし