METHOD FOR QUANTITATIVE DETECTION OF HUMAN CELLS

PROBLEM TO BE SOLVED: To provide a method for quantitative detection of human cells, the method suppressing many false positives detected due to a large number of copies of the Alu sequence in the genomic DNA when using a method for examining in vivo kinetics of human cells prepared for transplantat...

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Hauptverfasser:	TAKEDA RIE, ISHII TSUTOMU
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for quantitative detection of human cells, the method suppressing many false positives detected due to a large number of copies of the Alu sequence in the genomic DNA when using a method for examining in vivo kinetics of human cells prepared for transplantation by amplifying the Alu sequence as a target using a PCR method and quantifying the amount thereof.SOLUTION: According to the present invention, there is provided a method for quantitative detection of human cells, the method using a primer that amplifies the Alu-Yd6 sequence in a specific manner to solve the problem.SELECTED DRAWING: None 【課題】Ａｌｕ配列を標的としてＰＣＲ法を用いて増幅し、その量を定量することで、移植用に準備されたヒト細胞の動物内での体内動態を検査する方法では、Ａｌｕ配列のゲノムＤＮＡ内におけるコピー数が多いことから、偽陽性が多く検出される。そこで、当該検出方法を用いた場合の偽陽性を抑制することを課題とする。【解決手段】本発明は、Ａｌｕ-Ｙｄ６配列を特異的に増幅するプライマーを用いることで、当該課題を解決する。【選択図】なし