PRIMER AND METHOD FOR DETECTING MYCOPLASMA PNEUMONIAE
PROBLEM TO BE SOLVED: To provide detection methods in which Mycoplasma pneumoniae present in a sample is specifically amplified and false positivity is difficult to be generated.SOLUTION: The present invention provides a primer set which consists of a first primer having a sequence complementary to...
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| creator | TSUKAMOTO YU YOSHIOKA MIKI TAWARADA TAKAYA |
| description | PROBLEM TO BE SOLVED: To provide detection methods in which Mycoplasma pneumoniae present in a sample is specifically amplified and false positivity is difficult to be generated.SOLUTION: The present invention provides a primer set which consists of a first primer having a sequence complementary to the 3 'end portion of the specific base sequence of Mycoplasma pneumoniae 23S rRNA or 23S rDNA contained in a sample and a second primer having a sequence homologous to the 5 ' end portion of the specific base sequence, and which is for amplifying a nucleic acid comprising the specific base sequence or a complementary sequence of the specific base sequence, the first primer being an oligonucleotide capable of specifically hybridizing with a specific sequence under stringent conditions, and the second primer is an oligonucleotide capable of specifically hybridizing with a different specific sequence under stringent conditions; and a method of detecting Mycoplasma pneumoniae using the set.SELECTED DRAWING: None
【課題】試料中に存在するマイコプラズマ・ニューモニエを特異的に増幅し、かつ偽陽性が発生しにくい検出方法の提供。【解決手段】試料中に含まれるマイコプラズマ・ニューモニエ２３Ｓ ｒＲＮＡもしくは２３Ｓ ｒＤＮＡの特定塩基配列の３'末端部と相補的な配列を有する第一のプライマーと、前記特定塩基配列の５'末端部と相同的な配列を有する第二のプライマーとからなる、前記特定塩基配列または前記特定塩基配列の相補配列を含む核酸を増幅するためのプライマーセットであって、第一のプライマーが、特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドであり、第二のプライマーが、異なる特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドである、前記プライマーセット及び前記セットを用いて、マイコプラズマ・ニューモニエを検出する方法。【選択図】なし |
| format | Patent |
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【課題】試料中に存在するマイコプラズマ・ニューモニエを特異的に増幅し、かつ偽陽性が発生しにくい検出方法の提供。【解決手段】試料中に含まれるマイコプラズマ・ニューモニエ２３Ｓ ｒＲＮＡもしくは２３Ｓ ｒＤＮＡの特定塩基配列の３'末端部と相補的な配列を有する第一のプライマーと、前記特定塩基配列の５'末端部と相同的な配列を有する第二のプライマーとからなる、前記特定塩基配列または前記特定塩基配列の相補配列を含む核酸を増幅するためのプライマーセットであって、第一のプライマーが、特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドであり、第二のプライマーが、異なる特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドである、前記プライマーセット及び前記セットを用いて、マイコプラズマ・ニューモニエを検出する方法。【選択図】なし</description><language>eng ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170831&DB=EPODOC&CC=JP&NR=2017148008A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170831&DB=EPODOC&CC=JP&NR=2017148008A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TSUKAMOTO YU</creatorcontrib><creatorcontrib>YOSHIOKA MIKI</creatorcontrib><creatorcontrib>TAWARADA TAKAYA</creatorcontrib><title>PRIMER AND METHOD FOR DETECTING MYCOPLASMA PNEUMONIAE</title><description>PROBLEM TO BE SOLVED: To provide detection methods in which Mycoplasma pneumoniae present in a sample is specifically amplified and false positivity is difficult to be generated.SOLUTION: The present invention provides a primer set which consists of a first primer having a sequence complementary to the 3 'end portion of the specific base sequence of Mycoplasma pneumoniae 23S rRNA or 23S rDNA contained in a sample and a second primer having a sequence homologous to the 5 ' end portion of the specific base sequence, and which is for amplifying a nucleic acid comprising the specific base sequence or a complementary sequence of the specific base sequence, the first primer being an oligonucleotide capable of specifically hybridizing with a specific sequence under stringent conditions, and the second primer is an oligonucleotide capable of specifically hybridizing with a different specific sequence under stringent conditions; and a method of detecting Mycoplasma pneumoniae using the set.SELECTED DRAWING: None
【課題】試料中に存在するマイコプラズマ・ニューモニエを特異的に増幅し、かつ偽陽性が発生しにくい検出方法の提供。【解決手段】試料中に含まれるマイコプラズマ・ニューモニエ２３Ｓ ｒＲＮＡもしくは２３Ｓ ｒＤＮＡの特定塩基配列の３'末端部と相補的な配列を有する第一のプライマーと、前記特定塩基配列の５'末端部と相同的な配列を有する第二のプライマーとからなる、前記特定塩基配列または前記特定塩基配列の相補配列を含む核酸を増幅するためのプライマーセットであって、第一のプライマーが、特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドであり、第二のプライマーが、異なる特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドである、前記プライマーセット及び前記セットを用いて、マイコプラズマ・ニューモニエを検出する方法。【選択図】なし</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDANCPL0dQ1ScPRzUfB1DfHwd1Fw8w9ScHENcXUO8fRzV_CNdPYP8HEM9nVUCPBzDfX19_N0dOVhYE1LzClO5YXS3AxKbq4hzh66qQX58anFBYnJqXmpJfFeAUYGhuaGJhYGBhaOxkQpAgA3dCgW</recordid><startdate>20170831</startdate><enddate>20170831</enddate><creator>TSUKAMOTO YU</creator><creator>YOSHIOKA MIKI</creator><creator>TAWARADA TAKAYA</creator><scope>EVB</scope></search><sort><creationdate>20170831</creationdate><title>PRIMER AND METHOD FOR DETECTING MYCOPLASMA PNEUMONIAE</title><author>TSUKAMOTO YU ; YOSHIOKA MIKI ; TAWARADA TAKAYA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2017148008A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TSUKAMOTO YU</creatorcontrib><creatorcontrib>YOSHIOKA MIKI</creatorcontrib><creatorcontrib>TAWARADA TAKAYA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TSUKAMOTO YU</au><au>YOSHIOKA MIKI</au><au>TAWARADA TAKAYA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PRIMER AND METHOD FOR DETECTING MYCOPLASMA PNEUMONIAE</title><date>2017-08-31</date><risdate>2017</risdate><abstract>PROBLEM TO BE SOLVED: To provide detection methods in which Mycoplasma pneumoniae present in a sample is specifically amplified and false positivity is difficult to be generated.SOLUTION: The present invention provides a primer set which consists of a first primer having a sequence complementary to the 3 'end portion of the specific base sequence of Mycoplasma pneumoniae 23S rRNA or 23S rDNA contained in a sample and a second primer having a sequence homologous to the 5 ' end portion of the specific base sequence, and which is for amplifying a nucleic acid comprising the specific base sequence or a complementary sequence of the specific base sequence, the first primer being an oligonucleotide capable of specifically hybridizing with a specific sequence under stringent conditions, and the second primer is an oligonucleotide capable of specifically hybridizing with a different specific sequence under stringent conditions; and a method of detecting Mycoplasma pneumoniae using the set.SELECTED DRAWING: None
【課題】試料中に存在するマイコプラズマ・ニューモニエを特異的に増幅し、かつ偽陽性が発生しにくい検出方法の提供。【解決手段】試料中に含まれるマイコプラズマ・ニューモニエ２３Ｓ ｒＲＮＡもしくは２３Ｓ ｒＤＮＡの特定塩基配列の３'末端部と相補的な配列を有する第一のプライマーと、前記特定塩基配列の５'末端部と相同的な配列を有する第二のプライマーとからなる、前記特定塩基配列または前記特定塩基配列の相補配列を含む核酸を増幅するためのプライマーセットであって、第一のプライマーが、特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドであり、第二のプライマーが、異なる特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドである、前記プライマーセット及び前記セットを用いて、マイコプラズマ・ニューモニエを検出する方法。【選択図】なし</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | PRIMER AND METHOD FOR DETECTING MYCOPLASMA PNEUMONIAE |
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