PRIMER AND METHOD FOR DETECTING MYCOPLASMA PNEUMONIAE

PROBLEM TO BE SOLVED: To provide detection methods in which Mycoplasma pneumoniae present in a sample is specifically amplified and false positivity is difficult to be generated.SOLUTION: The present invention provides a primer set which consists of a first primer having a sequence complementary to...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: TSUKAMOTO YU, YOSHIOKA MIKI, TAWARADA TAKAYA
Format: Patent
Sprache:eng ; jpn
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:PROBLEM TO BE SOLVED: To provide detection methods in which Mycoplasma pneumoniae present in a sample is specifically amplified and false positivity is difficult to be generated.SOLUTION: The present invention provides a primer set which consists of a first primer having a sequence complementary to the 3 'end portion of the specific base sequence of Mycoplasma pneumoniae 23S rRNA or 23S rDNA contained in a sample and a second primer having a sequence homologous to the 5 ' end portion of the specific base sequence, and which is for amplifying a nucleic acid comprising the specific base sequence or a complementary sequence of the specific base sequence, the first primer being an oligonucleotide capable of specifically hybridizing with a specific sequence under stringent conditions, and the second primer is an oligonucleotide capable of specifically hybridizing with a different specific sequence under stringent conditions; and a method of detecting Mycoplasma pneumoniae using the set.SELECTED DRAWING: None 【課題】試料中に存在するマイコプラズマ・ニューモニエを特異的に増幅し、かつ偽陽性が発生しにくい検出方法の提供。【解決手段】試料中に含まれるマイコプラズマ・ニューモニエ23S rRNAもしくは23S rDNAの特定塩基配列の3'末端部と相補的な配列を有する第一のプライマーと、前記特定塩基配列の5'末端部と相同的な配列を有する第二のプライマーとからなる、前記特定塩基配列または前記特定塩基配列の相補配列を含む核酸を増幅するためのプライマーセットであって、第一のプライマーが、特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドであり、第二のプライマーが、異なる特定の配列とストリンジェントな条件で特異的にハイブリダイズ可能なオリゴヌクレオチドである、前記プライマーセット及び前記セットを用いて、マイコプラズマ・ニューモニエを検出する方法。【選択図】なし