CIRCUIT FOR ELECTROPORATION

PROBLEM TO BE SOLVED: To provide a circuit for electroporation that realizes higher viability in sending DNA into a cell to perform transformation.SOLUTION: A circuit 2 for electroporation applies DC pulse to a buffer containing a cell via a pair of load terminals 12, 14 to perform introduction. In...

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1. Verfasser:	MORIIZUMI TOSHIYUKI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BASIC ELECTRONIC CIRCUITRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ELECTRICITY ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PULSE TECHNIQUE SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a circuit for electroporation that realizes higher viability in sending DNA into a cell to perform transformation.SOLUTION: A circuit 2 for electroporation applies DC pulse to a buffer containing a cell via a pair of load terminals 12, 14 to perform introduction. In electroporation, the circuit 2 includes a signal generator 4 and switching elements Q1, Q2 which perform short circuit the pair of load terminals 12, 14 on turning to an ON-state. The signal generator 4 gives a signal for turning the switching elements Q1, Q2 to an ON-state to the switching elements Q1,Q2 in all or a part of a period when DC pulse is not applied.SELECTED DRAWING: Figure 1 【課題】ＤＮＡを細胞内部に送り込んで形質転換に当って、より高いバイアビリティを実現する、エレクトロポレーションのための回路の提供。【解決手段】一対の負荷端子１２，１４を介して、細胞を含むバッファに直流パルスを印加して導入を行うエレクトロポレーションのための回路２。エレクトロポレーションは、シグナルジェネレータ４と、オン状態となることにより一対の負荷端子１２，１４を短絡するスイッチング素子Ｑ１，Ｑ２とを含む回路２。シグナルジェネレータ４は、直流パルスを印加しないときの全て又は一部にて、スイッチング素子Ｑ１，Ｑ２をオン状態とする信号をスイッチング素子Ｑ１，Ｑ２に与える回路２。【選択図】図１