BACILLUS SUBTILIS MUTANTS AND METHODS FOR PRODUCING DIPICOLINIC ACID USING THE SAME

BACILLUS SUBTILIS MUTANTS AND METHODS FOR PRODUCING DIPICOLINIC ACID USING THE SAME

PROBLEM TO BE SOLVED: To provide recombinant microorganisms capable of producing dipicolinic acid with high yield.SOLUTION: The invention provides a mutant of Bacillus subtilis in which, at least one region selected from prophage region, PBSX region, spb region, pks region, skin region, pps region,...

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Bibliographische Detailangaben
Hauptverfasser: RYU SEIKO, MASUDA KENTA
Format: Patent
Sprache:eng ; jpn
Schlagworte:
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BIOCHEMISTRY
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CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
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Beschreibung
Zusammenfassung:PROBLEM TO BE SOLVED: To provide recombinant microorganisms capable of producing dipicolinic acid with high yield.SOLUTION: The invention provides a mutant of Bacillus subtilis in which, at least one region selected from prophage region, PBSX region, spb region, pks region, skin region, pps region, ydcL-ydeK-ydhU region, yisB-yitD region, yunA-yurT region, cgeE-ypmQ region, yeeK-yesX region, pdp-rocR region, ycxB-sipU region, SKIN-Pro7 region, sbo-ywhH region, yybP-yyaJ region, and yncM-fosB region is deleted from the genome, the expression of B. subtilis dipicolinic acid synthase gene is enhanced, and at least one gene selected from alsS and alsD, and ackA and pta genes is deleted or inactivated.SELECTED DRAWING: None 【課題】ジピコリン酸を高生産することができる組換え微生物の提供。【解決手段】枯草菌変異株であって、ｐｒｏｐｈａｇｅ領域、ＰＢＳＸ領域、ｓｐｂ領域、ｐｋｓ領域、ｓｋｉｎ領域、ｐｐｓ領域、ｙｄｃＬ−ｙｄｅＫ−ｙｄｈＵ領域、ｙｉｓＢ−ｙｉｔＤ領域、ｙｕｎＡ−ｙｕｒＴ領域、ｃｇｅＥ−ｙｐｍＱ領域、ｙｅｅＫ−ｙｅｓＸ領域、ｐｄｐ−ｒｏｃＲ領域、ｙｃｘＢ−ｓｉｐＵ領域、ＳＫＩＮ−Ｐｒｏ７領域、ｓｂｏ−ｙｗｈＨ領域、ｙｙｂＰ−ｙｙａＪ領域及びｙｎｃＭ−ｆｏｓＢ領域より選択される１以上の領域が欠失したゲノムを有し、枯草菌ジピコリン酸シンターゼ遺伝子の発現が強化されており、且つ、ａｌｓＳ遺伝子及びａｌｓＤ遺伝子、及び、ａｃｋＡ遺伝子及びｐｔａ遺伝子より選択される少なくとも１つの遺伝子が欠失又は不活性化されている枯草菌変異株。【選択図】なし