EXPRESSION METHOD
PROBLEM TO BE SOLVED: To provide production methods by which the product yield of a protein can be enhanced in microbial fermentation, to provide a microorganism obtained by the method, and to provide use of the microorganism for protein production.SOLUTION: The present invention provides a method o...
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| creator | RAMONA KNAB LUKAS MAKSYM JOHANNES BONGAERTS STEFAN EVERS KARL-HEINZ MAURER |
| description | PROBLEM TO BE SOLVED: To provide production methods by which the product yield of a protein can be enhanced in microbial fermentation, to provide a microorganism obtained by the method, and to provide use of the microorganism for protein production.SOLUTION: The present invention provides a method of producing a protein by a microorganism, wherein in addition to the first expression construct encoding the protein, a second expression construct which, unlike the protein, has proteolytic activity and which encodes boosted protease containing an amino acid sequence at least 50% identical to the amino acid sequence having a particular sequence is introduced into a microorganism to be expressed. The protein and/or the boosted protease is not present in the microorganism naturally.SELECTED DRAWING: None
【課題】微生物発酵において、タンパク質の生成物収率が高められる製造方法、当該方法で得られる微生物、及び、タンパク質製造のための当該微生物の使用の提供。【解決手段】タンパク質をコードする第１の発現コンストラクトの他に、前記タンパク質とは異なり、タンパク質分解活性があり、特定の配列を有するアミノ酸配列と少なくとも５０％同一であるアミノ酸配列を含む補助プロテアーゼをコードする第２発現コンストラクトを微生物中に導入し、発現させて、微生物によるタンパク質を製造する方法。前記タンパク質及び／又は前記補助プロテアーゼは前記微生物中には天然に存在しない、タンパク質の製造方法。【選択図】なし |
| format | Patent |
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【課題】微生物発酵において、タンパク質の生成物収率が高められる製造方法、当該方法で得られる微生物、及び、タンパク質製造のための当該微生物の使用の提供。【解決手段】タンパク質をコードする第１の発現コンストラクトの他に、前記タンパク質とは異なり、タンパク質分解活性があり、特定の配列を有するアミノ酸配列と少なくとも５０％同一であるアミノ酸配列を含む補助プロテアーゼをコードする第２発現コンストラクトを微生物中に導入し、発現させて、微生物によるタンパク質を製造する方法。前記タンパク質及び／又は前記補助プロテアーゼは前記微生物中には天然に存在しない、タンパク質の製造方法。【選択図】なし</description><language>eng ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170518&DB=EPODOC&CC=JP&NR=2017079768A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170518&DB=EPODOC&CC=JP&NR=2017079768A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>RAMONA KNAB</creatorcontrib><creatorcontrib>LUKAS MAKSYM</creatorcontrib><creatorcontrib>JOHANNES BONGAERTS</creatorcontrib><creatorcontrib>STEFAN EVERS</creatorcontrib><creatorcontrib>KARL-HEINZ MAURER</creatorcontrib><title>EXPRESSION METHOD</title><description>PROBLEM TO BE SOLVED: To provide production methods by which the product yield of a protein can be enhanced in microbial fermentation, to provide a microorganism obtained by the method, and to provide use of the microorganism for protein production.SOLUTION: The present invention provides a method of producing a protein by a microorganism, wherein in addition to the first expression construct encoding the protein, a second expression construct which, unlike the protein, has proteolytic activity and which encodes boosted protease containing an amino acid sequence at least 50% identical to the amino acid sequence having a particular sequence is introduced into a microorganism to be expressed. The protein and/or the boosted protease is not present in the microorganism naturally.SELECTED DRAWING: None
【課題】微生物発酵において、タンパク質の生成物収率が高められる製造方法、当該方法で得られる微生物、及び、タンパク質製造のための当該微生物の使用の提供。【解決手段】タンパク質をコードする第１の発現コンストラクトの他に、前記タンパク質とは異なり、タンパク質分解活性があり、特定の配列を有するアミノ酸配列と少なくとも５０％同一であるアミノ酸配列を含む補助プロテアーゼをコードする第２発現コンストラクトを微生物中に導入し、発現させて、微生物によるタンパク質を製造する方法。前記タンパク質及び／又は前記補助プロテアーゼは前記微生物中には天然に存在しない、タンパク質の製造方法。【選択図】なし</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZBB0jQgIcg0O9vT3U_B1DfHwd-FhYE1LzClO5YXS3AxKbq4hzh66qQX58anFBYnJqXmpJfFeAUYGhuYG5pbmZhaOxkQpAgBsfB57</recordid><startdate>20170518</startdate><enddate>20170518</enddate><creator>RAMONA KNAB</creator><creator>LUKAS MAKSYM</creator><creator>JOHANNES BONGAERTS</creator><creator>STEFAN EVERS</creator><creator>KARL-HEINZ MAURER</creator><scope>EVB</scope></search><sort><creationdate>20170518</creationdate><title>EXPRESSION METHOD</title><author>RAMONA KNAB ; LUKAS MAKSYM ; JOHANNES BONGAERTS ; STEFAN EVERS ; KARL-HEINZ MAURER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JP2017079768A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; jpn</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>RAMONA KNAB</creatorcontrib><creatorcontrib>LUKAS MAKSYM</creatorcontrib><creatorcontrib>JOHANNES BONGAERTS</creatorcontrib><creatorcontrib>STEFAN EVERS</creatorcontrib><creatorcontrib>KARL-HEINZ MAURER</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>RAMONA KNAB</au><au>LUKAS MAKSYM</au><au>JOHANNES BONGAERTS</au><au>STEFAN EVERS</au><au>KARL-HEINZ MAURER</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>EXPRESSION METHOD</title><date>2017-05-18</date><risdate>2017</risdate><abstract>PROBLEM TO BE SOLVED: To provide production methods by which the product yield of a protein can be enhanced in microbial fermentation, to provide a microorganism obtained by the method, and to provide use of the microorganism for protein production.SOLUTION: The present invention provides a method of producing a protein by a microorganism, wherein in addition to the first expression construct encoding the protein, a second expression construct which, unlike the protein, has proteolytic activity and which encodes boosted protease containing an amino acid sequence at least 50% identical to the amino acid sequence having a particular sequence is introduced into a microorganism to be expressed. The protein and/or the boosted protease is not present in the microorganism naturally.SELECTED DRAWING: None
【課題】微生物発酵において、タンパク質の生成物収率が高められる製造方法、当該方法で得られる微生物、及び、タンパク質製造のための当該微生物の使用の提供。【解決手段】タンパク質をコードする第１の発現コンストラクトの他に、前記タンパク質とは異なり、タンパク質分解活性があり、特定の配列を有するアミノ酸配列と少なくとも５０％同一であるアミノ酸配列を含む補助プロテアーゼをコードする第２発現コンストラクトを微生物中に導入し、発現させて、微生物によるタンパク質を製造する方法。前記タンパク質及び／又は前記補助プロテアーゼは前記微生物中には天然に存在しない、タンパク質の製造方法。【選択図】なし</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | EXPRESSION METHOD |
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