BIOTIN VARIANT, STREPTAVIDIN MUTANT, AND USE THEREOF

PROBLEM TO BE SOLVED: To provide a streptavidin mutant that has reduced affinity for natural biotin, and further to provide a biotin variant that has high affinity for the streptavidin mutant having low affinity for natural biotin.SOLUTION: According to the present invention, there is provided a com...

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Hauptverfasser:	IIZUKA TOMOHIRO, KAWATO TATSUYA, SUGIYAMA AKIRA, DOI HIROFUMI, INOUE TAKESHI, TAKASU NORIAKI, TAKATSU MARI, MIZOHATA EIICHI, KODAMA TATSUHIKO, KANAI MOTOMU, SHIMIZU YOHEI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HETEROCYCLIC COMPOUNDS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a streptavidin mutant that has reduced affinity for natural biotin, and further to provide a biotin variant that has high affinity for the streptavidin mutant having low affinity for natural biotin.SOLUTION: According to the present invention, there is provided a compound comprising a dimer of a biotin variant, a streptavidin mutant, and use thereof.SELECTED DRAWING: None 【課題】本発明の課題は、天然ビオチンに対する親和性を低減させたストレプトアビジン変異体を提供し、更にこの天然ビオチンに対する低親和性のストレプトアビジン変異体に対して高い親和性を有するビオチン改変体を提供することである。【解決手段】本発明によれば、ビオチン改変体の２量体からなる化合物、ストレプトアビジン変異体、並びにそれらの利用が提供される。【選択図】なし