NOVEL MANNANASE AND NOVEL MICROORGANISM PRODUCING THIS

PROBLEM TO BE SOLVED: To provide (1) novel mannanase by which mannobiose can be obtained in high yield, the mannanase producing extremely little amount of monosaccharides (mannose and the like) even if hydrolyzing mannan, and to provide (2) a microorganism which produces such mannanase.SOLUTION: The...

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Hauptverfasser:	YOSHIDA YASUHIKO, TSUMURA KAZUNOBU, KURE SHOJI, NAKAMURA AKIHIRO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	PROBLEM TO BE SOLVED: To provide (1) novel mannanase by which mannobiose can be obtained in high yield, the mannanase producing extremely little amount of monosaccharides (mannose and the like) even if hydrolyzing mannan, and to provide (2) a microorganism which produces such mannanase.SOLUTION: The present invention provides mannanase MN-1 having the following physicochemical properties. 1) Action: acting on mannan to preferentially produce mannobiose. 2) Substrate specificity : hydrolyzing β-1,4 mannopyranoside binding. 3) Stable pH of 4-7. 4) Acting temperature of 30-50°C. 5) Temperature stability in 50°C or less. 6) Molecular weight of 40,000. Bacillus amyloliquefaciens MN47 (NITE P-01816) belongs to Bacillus and produces a mannanase.SELECTED DRAWING: None 【課題】(1)マンナンを加水分解しても単糖（マンノース等）の生成が極めて少なく、高収率でマンノビオースを得ることができる新規なマンナナーゼ及び(2)かかるマンナナーゼを産生する微生物。【解決手段】次の理化学的性質を有するマンナナーゼＭＮ−１ 1）作用：マンナンに作用してマンノビオースを優先的に生成する。2）基質特異性：β−１，４マンノピラノシド結合を加水分解する。3）安定ｐＨは４〜７。4）作用温度は３０〜５０℃。5）温度安定性は５０℃以下。6）分子量は４万。バチルス属に属しマンナナーゼを産生するバチルス・アミロリケファシエンスＭＮ４７（ＮＩＴＥＰ−０１８１６）。【選択図】なし