OLIGONUCLEOTIDE PRIMERS FOR DETECTING NEISSERIA GONORRHOEAE AND METHODS FOR DETECTING NEISSERIA GONORRHOEAE USING THE PRIMERS

PROBLEM TO BE SOLVED: To provide oligonucleotide primers for specifically detecting Neisseria gonorrhoeae in a sample and methods for detecting Neisseria gonorrhoeae using the primers.SOLUTION: A primer set comprises a first primer having a sequence homologous to a part of a specified base sequence...

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Hauptverfasser:	UNE KURATO, KITAMORI YUKA, TAWARADA TAKAYA
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide oligonucleotide primers for specifically detecting Neisseria gonorrhoeae in a sample and methods for detecting Neisseria gonorrhoeae using the primers.SOLUTION: A primer set comprises a first primer having a sequence homologous to a part of a specified base sequence of gonococcal 16S rRNA in a sample, and a second primer having a sequence complementary to a part of the specified base sequence, where the primer set is optimized to amplify a nucleic acid comprising the specified base sequence or a complementary strand of the specified base sequence.SELECTED DRAWING: None 【課題】試料中に存在する淋菌を特異的に検出するためのオリゴヌクレオチドプライマー、および当該プライマーを用いた淋菌の検出方法を提供すること。【解決手段】試料中に含まれる淋菌１６ＳｒＲＮＡの特定塩基配列の一部と相同的な配列を有する第一のプライマーと、前記特定塩基配列の一部と相補的な配列を有する第二のプライマーとからなる、前記特定塩基配列または前記特定塩基配列の相補鎖を含む核酸を増幅するためのプライマーセットを最適化することで、前記課題を解決する。【選択図】なし