INSTRUMENT FOR MONITORING POLYMERASE CHAIN REACTION OF DNA

PROBLEM TO BE SOLVED: To provide: a novel optical instrument for quantitative monitoring of DNA replication in a PCR apparatus; and such an instrument with an improved dynamic range, automatic selection of exposure time to extend the dynamic range, automatic adjustment for drift, simplified operatio...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LAKATOS EDWARD J, GAMBINI MICHAEL R, YOUNG EUGENE F, CERRONE ANTHONY L, ATWOOD JOHN G
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING TRANSPORTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide: a novel optical instrument for quantitative monitoring of DNA replication in a PCR apparatus; and such an instrument with an improved dynamic range, automatic selection of exposure time to extend the dynamic range, automatic adjustment for drift, simplified operation, relatively low cost, and easy changing of optics to accommodate different fluorescent dyes.SOLUTION: The invention provides filter modules for an optical instrument for monitoring polymerase chain reaction replication of DNA in a reaction apparatus that includes a thermal cycler block for holding a plurality of vials containing a suspension of ingredients for the reaction.SELECTED DRAWING: None 【課題】ＰＣＲ装置でのＤＮＡ複製の定量モニタリング用の新規な光学器械を提供すること、およびダイナミックレンジが改善され、ダイナミックレンジを拡大する露光時間を自動で選択でき、ドリフトが自動で調整され、操作が容易であり、相対的に低価格で、異なる蛍光染料を収納するのに光学系の変更が簡単な上記機器を提供すること。【解決手段】反応用の成分の懸濁液を含んでいる複数の小びんを保持する熱サイクルブロックを含む反応装置のＤＮＡのポリメラーゼ連鎖反応複製をモニタするための光学機器用のフィルタモジュール。【選択図】なし