MUTANT GLYCOSIDE HYDROLASES

PROBLEM TO BE SOLVED: To provide mutant glycoside hydrolases with high heat resistance and high stability in liquid detergents, in particular, excellent stability to surfactants.SOLUTION: The mutant glycoside hydrolase comprises a specified amino acid sequence or an amino acid sequence having 80% or...

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Bibliographische Detailangaben
1. Verfasser:	RYU SEIKO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide mutant glycoside hydrolases with high heat resistance and high stability in liquid detergents, in particular, excellent stability to surfactants.SOLUTION: The mutant glycoside hydrolase comprises a specified amino acid sequence or an amino acid sequence having 80% or more sequence identity to the specified amino acid sequence, where the amino acid residues at the positions 138, 301 and 310 or at positions corresponding to these positions are: (a) at position 138 or corresponding position: histidine; (b) at position 301 or corresponding position: histidine; and (c) at position 310 or at corresponding position: glutamic acid.SELECTED DRAWING: None 【課題】耐熱性が優れ、且つ液体洗剤中での安定性、特に界面活性剤に対して優れた安定性を有する変異グリコシドハイドロラーゼの提供。【解決手段】特定のアミノ酸配列又は前記アミノ酸配列と８０％以上の配列同一性を有するアミノ酸配列において、１３８位、３０１位及び３１０位又はこれらに相当する位置におけるアミノ酸残基が下記のアミノ酸残基である変異グリコシドハイドロラーゼ。（ａ）１３８位又はこれらに相当する位置：ヒスチジン（ｂ）３０１位又はこれらに相当する位置：ヒスチジン（ｃ）３１０位又はこれらに相当する位置：グルタミン酸【選択図】なし