CELL DETECTION WITH CONJUGATES HAVING ENZYMATICALLY RELEASABLE DETECTION MOIETY

PROBLEM TO BE SOLVED: To provide a method for specific labelling and detection of a target in or on a sample of a biological specimen and subsequently removing the detection moiety in order to provide reversible labelling and/or enable further different labelling and detection cycles.SOLUTION: This...

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Bibliographische Detailangaben
Hauptverfasser:	CHRISTIAN DOSE, MILTENYI STEFAN, JENNIFER BRIEDEN, VERONIKA RUDOLF, THOMAS ROCKEL
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for specific labelling and detection of a target in or on a sample of a biological specimen and subsequently removing the detection moiety in order to provide reversible labelling and/or enable further different labelling and detection cycles.SOLUTION: This invention relates to a method for detecting a target moiety in a sample of biological specimens by: a) providing at least one conjugate having an enzymatically releasable degradable spacer, where the detection moiety and an antigen recognizing moiety are covalently bound; b) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety; c) detecting the target moiety labeled with the conjugate with the detecting moiety; and d) enzymatically degrading spacer, thereby cleaving the detection moiety from the conjugate.SELECTED DRAWING: None 【課題】生物学的検体の試料中または試料上で標的を特異的に標識および検出し、引き続き検出部を除去して、可逆的な標識をもたらし、及び／又は更なる異なる標識および検出のサイクルを可能にする方法の提供。【解決手段】ａ）前記検出部と抗原認識部とが共有結合されている、酵素的に分解可能なスペーサーを有する少なくとも１種の接合体を準備すること、ｂ）前記生物学的検体の試料と少なくとも１種の前記接合体とを接触させることで、前記抗原認識部によって認識される標的部を標識すること、ｃ）前記結合部で標識された前記標的部を、前記検出部により検出すること、並びに、ｄ）前記スペーサーを酵素的に分解することで、前記検出部を前記接合体から開裂させること、によって行う生物学的検体の試料中の標的部を検出するための方法。【選択図】なし