CELL CULTURE SUBSTRATE HAVING TWO TYPES OF POROUS FILM WITH DIFFERENT PORE SIZES, AND CELL CULTURE TOOL HAVING THE SAME

PROBLEM TO BE SOLVED: To provide means, in co-culture using a feeder cell, for substantially preventing the feeder cell or its cell residue from being mixed into a target cell without inhibiting a growth promotion effect for the target cell.SOLUTION: The present invention relates to a cell culture tool has: at least one cell culture substrate which has a first film having a plurality of pores and a second film having a plurality of pores, in which the second film is arranged on a side used for cell culture and the first film is arranged on the opposite side to the side used for cell culture with respect to the second film, and a pore size of a plurality of pores of the first film is a pore size or less of a plurality of pores of the second film; and a cell culture vessel inside of which at least the one cell culture substrate is arranged.SELECTED DRAWING: Figure 1 【課題】フィーダー細胞を用いる共培養において、対象細胞に対する生育促進効果を阻害することなく、フィーダー細胞又はその細胞残渣が対象細胞に混入することを実質的に防止し得る手段を提供する。【解決手段】本発明は、複数の孔を有する第1の膜と複数の孔を有する第2の膜とを有し、第2の膜が細胞培養に使用される側に、第1の膜が第2の膜に対して細胞培養に使用される側とは反対側にそれぞれ配置されており、第1の膜の複数の孔の孔径が第2の膜の複数の孔の孔径以下である、少なくとも1個の細胞培養基材と、該少なくとも1個の細胞培養基材が内部に配置された細胞培養容器とを有する、細胞培養器具に関する。【選択図】図１